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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,044 results for: ICL

Showing: 341 - 360 of 3044 results

  2. pGAPTrap TALEN 2

    Plasmid
    #83369
    Purpose
    Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    GAPDH_TALEN2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pGAPTrap-TALEN1

    Plasmid
    #83368
    Purpose
    Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    GAPDH_TALEN1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. K15-EGFP

    Plasmid
    #44266
    Depositor
    George Cotsarelis
    Article
    Morris et al Nat Biotechnol. 2004 Apr;22(4):411-7. Ep
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Mouse Targeting; Fluorescence microscopy
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pGAPTrap-GFP

    Plasmid
    #82506
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82506-plasmid-map-sequence-id-156571

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  12. RGD-ACP-I27-CC

    Plasmid
    #85391
    Purpose
    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and ACP tag for fluorophore labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to acyl carrier protein (ACP) tag and titin immunoglobulin domain (I27)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85391-plasmid-map-sequence-id-172701

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  13. K15/pGL3

    Plasmid
    #44264
    Depositor
    George Cotsarelis
    Article
    Liu et al J Invest Dermatol. 2003 Nov;121(5):963-8
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Luciferase and Mouse Targeting
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. FN(9-10, RGE)-I27-CC

    Plasmid
    #85396
    Purpose
    MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    GRGDS motif mutated to GRGES
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85396-plasmid-map-sequence-id-172700

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  16. CROPseq-Puro-24xMS2

    Plasmid
    #212629
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212629-plasmid-map-sequence-id-420222

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  17. GFP-noPS

    Plasmid
    #177943
    Purpose
    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    177943-plasmid-map-sequence-id-350212

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  18. Luc-noPS

    Plasmid
    #177940
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177940-plasmid-map-sequence-id-348112

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  19. KG#901

    Plasmid
    #110936
    Purpose
    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110936-plasmid-map-sequence-id-216868

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Showing: 341 - 360 of 3044 results