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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0623

Plasmid

#128998

Purpose

pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pC.059

Plasmid

#119564

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Ptrc10

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.122

Plasmid

#119629

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

sgRNA scaffold

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.203

Plasmid

#119604

Purpose

Level 0 part. TSS promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23103_TSS

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.082

Plasmid

#119611

Purpose

Level 0 part. Terminator

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TrrnB

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.029

Plasmid

#119626

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbREry

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.078

Plasmid

#119609

Purpose

Level 0 part. Terminator

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Tcpc_operon

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.083

Plasmid

#119592

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23119MH_V01

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEM352

Plasmid

#248764

Purpose

160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-160xTetO

Expression

Bacterial

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pAH119

Plasmid

#248732

Purpose

PBT0479 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0479-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pEM547

Plasmid

#248742

Purpose

PBT0779 biosensor circuit (PTetR56 version) in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0779-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAH120

Plasmid

#248733

Purpose

PBT1004 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT1004-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAH108

Plasmid

#248728

Purpose

PBT0463 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0463-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAH110

Plasmid

#248730

Purpose

PBT0722 biosensor circuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT0722-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEM413

Plasmid

#248765

Purpose

10xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-10xTetO

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEM124

Plasmid

#248735

Purpose

Entry Vector for inserting Inducible promoters upstream of sfGFP in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

[pJ2300-PETRBS-mCherry-rrnBT1 dropout

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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