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We narrowed to 1,112 results for: cas12a

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Showing: 341 - 360 of 1112 results

  2. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86619-plasmid-map-sequence-id-168855

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  5. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid
    #114094
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R and K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114094-plasmid-map-sequence-id-220561

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  6. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid
    #114091
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a (E174R/S542R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R and S542R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114091-plasmid-map-sequence-id-220538

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  12. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid
    #114086
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114086-plasmid-map-sequence-id-231034

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  20. pTE4565

    Plasmid
    #88903
    Purpose
    Expresses inactive/dead, humanized MbCpf1 nuclease
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hMbCpf1(D986A)
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    88903-plasmid-map-sequence-id-172594

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Showing: 341 - 360 of 1112 results