We narrowed to 162,055 results for: ins;

1. insulator_seq_reporter_vector

   Plasmid

   #221460

   Purpose

   Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.

   Depositor

   Maria Cristina Gambetta

   Article

   Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

   Insert

   gypsy insulator-EGFP-neutral spacer DNA-mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINSPECT_SigP:NLuc

   Plasmid

   #197263

   Purpose

   Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM9121_TwinStrep

   Plasmid

   #153513

   Purpose

   TwinStrep C-terminal Tag Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   TwinStrep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM_HcBOP1ainsitu

   Plasmid

   #136529

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Houttuynia cordata BLADE-ON-PETIOLE homolog1a
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM_ZmaBOP1binsitu

   Plasmid

   #136531

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Zoysia matrella BLADE-ON-PETIOLE homolog1b
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM_ZmaBOP2binsitu

   Plasmid

   #136532

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Zoysia matrella BLADE-ON-PETIOLE homolog2b
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM_HcBOP1binsitu

   Plasmid

   #136530

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Houttuynia cordata BLADE-ON-PETIOLE homolog1b
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_RIT1_p.T76insTDLT

   Plasmid

   #82916

   Purpose

   Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RIT1 (RIT1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   TDLT insertion at residue T76

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

10. Jun lab tunable CRISPRi strain

    Bacterial Strain

    #86400

    Purpose

    Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

    Depositor

    Suckjoon Jun

    Article

    Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

    Bacterial Resistance

    Spectinomycin and hygromycin

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

11. BW-Para

    Bacterial Strain

    #172602

    Purpose

    The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Bacterial Resistance

    None

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

12. ML2

    Bacterial Strain

    #61906

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    Kanamycin

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. ins:MCS2; cryaa:RFP

    Plasmid

    #110286

    Purpose

    A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Type

    Empty backbone

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    insulin

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. InsRA-eGFP

    Plasmid

    #79795

    Purpose

    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Use

    Tags

    eGFP extracellular

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TMC1-TwinStrepII

    Plasmid

    #83267

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    TMC1 (Tmc1 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-calpainsensor

    Plasmid

    #36182

    Purpose

    detection of activity of ubiquitous calpains

    Depositor

    Isabelle Richard

    Article

    Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

    Insert

    alpha-Fodrin cleavage site (Sptan1 Mouse)
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. RINS1(mut)

    Plasmid

    #107291

    Purpose

    Plasmid for the expression of the inactive mutant of RINS1 sensor

    Depositor

    Dmytro Yushchenko

    Article

    Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

    Insert

    RINS1(mut)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    two Arg/Ser mutations at the beginning and in the…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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