We narrowed to 21,537 results for: mpl

1. pORANGE LOX Cloning template vector

   Plasmid

   #139651

   Purpose

   Cloning template for ORANGE-CAKE conditional knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. hU6-pegRNA-EFS-mScarlet Template

   Plasmid

   #196037

   Purpose

   The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and EFS

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

   Plasmid

   #70658

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pBx1-VHH template1-3XMyc-Spacer

   Plasmid

   #176208

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template1-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBx1-VHH template3-3XMyc-Spacer

   Plasmid

   #176210

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template3-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBx1-VHH template2-3XMyc-Spacer

   Plasmid

   #176209

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template2-3XMyc-Spacer
   

   Use

   Tags

   Myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. human SLC12A5-V5-HA-2A-Luciferase repair template

   Plasmid

   #158576

   Purpose

   For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
   

   Use

   Tags

   V5 and HA on the endogenous KCC2 protein product

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

   Plasmid

   #70659

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHnS KI;Actb Donor;3xV5 KO;Template

   Plasmid

   #240291

   Purpose

   KI:Actb Donor:3xV5 KO:Template

   Depositor

   Yuki Ogawa

   Insert

   KI gRNA for Actb
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHnS KI;Sptbn4 Donor;3xV5 KO;Template

    Plasmid

    #240295

    Purpose

    KI:Sptbn4 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Sptbn4
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHnS KI;Gria1 Donor;3xV5 KO;Template

    Plasmid

    #240293

    Purpose

    KI:Gria1 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Gria1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHnS KI;Pdha1 Donor;3xV5 KO;Template

    Plasmid

    #240300

    Purpose

    KI:Pdha1 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Pdha1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHnS KI;Lmnb1 Donor;3xV5 KO;Template

    Plasmid

    #240297

    Purpose

    KI:Lmnb1 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Lmnnb1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19_msfGFP-NPM1-repair-template for U2OS

    Plasmid

    #238246

    Purpose

    For knock-in msfGFP to NPM1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19_msfGFP-NPM1-repair-template for HCT116

    Plasmid

    #238245

    Purpose

    For knock-in msfGFP to NPM1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

    Plasmid

    #235608

    Purpose

    Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD027 Reporter Template v1 (Near Spaced)

    Plasmid

    #138751

    Purpose

    Template for COMET promoters, expresses EYFP reporter upon promoter activation.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD032 Reporter Template v1 (Mid Spaced)

    Plasmid

    #138753

    Purpose

    Template for COMET promoters, expresses EYFP reporter upon promoter activation.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD033 Reporter Template v1 (Far Spaced)

    Plasmid

    #138754

    Purpose

    Template for COMET promoters, expresses EYFP reporter upon promoter activation.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47868

    Purpose

    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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