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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 341 - 360 of 6562 results

  1. pSI-Target-AID-NG

    Plasmid
    #119861
    Purpose
    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.
    Depositor
    Osamu Nureki
    Publication
    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    Expression
    Mammalian
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    119861-plasmid-map-sequence-id-232065

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  3. pcDNA3-RAB-EKARev-NLS

    Plasmid
    #118476
    Purpose
    ddRFP-based single-color ERK biosensor targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev-NLS
    Tags
    6xHIS, Nuclear localization signal (NLS), T7 tag …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    118476-plasmid-map-sequence-id-243128

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  8. pRD438

    Plasmid
    #168464
    Purpose
    For the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mEos3.2-NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168464-plasmid-map-sequence-id-331762

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  13. pCSN074

    Plasmid
    #166731
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166731-plasmid-map-sequence-id-332203

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  16. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  17. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65769-plasmid-map-sequence-id-112659

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  20. pCS2+HyPer7-NLS

    Plasmid
    #136468
    Purpose
    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    Triple nuclear localization signal of SV40 (simia…
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    136468-plasmid-map-sequence-id-308715

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Showing: 341 - 360 of 6562 results