We narrowed to 4,430 results for: Erf

1. pJMP1189

   Plasmid

   #119257

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL Hygro-NFLAG TRF1 deltaA

   Plasmid

   #16278

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted acidic domain (aa1-66)

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pWZL Hygro-NFLAG TRF1 deltaM

   Plasmid

   #16281

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted myb domain (aa379-439)

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. HiFi dCas9 KRAB

   Plasmid

   #188500

   Purpose

   Expresses FLAG tagged HiFi dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCSG28-SH3-588

   Plasmid

   #185920

   Purpose

   Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

   Depositor

   Brian Kuhlman

   Article

   Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

   Insert

   SH3-588
   

   Use

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Designed enzyme with novel interface between huma…

   Promoter

   T7

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1223

   Plasmid

   #119261

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMD19T-psba1-TetR-PL22-dCas9-SpR

   Plasmid

   #73223

   Purpose

   Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PL22

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP1335

   Plasmid

   #119269

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Use

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP1363

    Plasmid

    #119279

    Purpose

    for stabilizing ICE in the presence of rapI expression

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-sfGFP[N150TAA]

    Plasmid

    #164581

    Purpose

    superfolder GFP with N150TAA mutation

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N150TAA Mutation

    Promoter

    T7

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDN0606 (pDEST-DHFR F[3] N-term,HygR)

    Plasmid

    #210488

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

    Plasmid

    #210485

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

    Plasmid

    #210487

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKB12 (pDEST-DHFR F[3] C-term, HygR)

    Plasmid

    #210486

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

    Plasmid

    #167877

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_A0402

    Plasmid

    #91009

    Purpose

    Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    AtUbi10

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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