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pEGFPC2-gephyrin S268A/270A

Plasmid

#199608

Purpose

Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

S268A/S270A

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAGE2.0

Plasmid

#165984

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin E

Plasmid

#68819

Purpose

Flag tagged mammalian expression of E domain

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

internal EcoRI site has silent mutation

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLL5.0 ShCavin1-EGFP

Plasmid

#187253

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AT5G48740 O3_pECIA2

Plasmid

#114963

Purpose

Bait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G48740 (AT5G48740 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT5G59650 O4_pECIA2

Plasmid

#114964

Purpose

Bait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G59650 (AT5G59650 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pTBL472

Plasmid

#126457

Purpose

Expresses Cas9-10xFlag-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

Cas9
10x Flag
TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G56120 X070_pECIA14

Plasmid

#114860

Purpose

Prey vector AT1G56120 X070_pECIA14 should be used with bait vector AT1G56120 X070_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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AT1G56120 X070_pECIA2

Plasmid

#115060

Purpose

Bait vector AT1G56120 X070_pECIA2 should be used with prey vector AT1G56120 X070_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56120 (AT1G56120 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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PXY X058_pECIA2

Plasmid

#115115

Purpose

Bait vector PXY X058_pECIA2 should be used with prey vector PXY X058_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G61480

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G24130 L4_pECIA2

Plasmid

#115121

Purpose

Bait vector AT2G24130 L4_pECIA2 should be used with prey vector AT2G24130 L4_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G24130 (AT2G24130 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT3G47090 L5_pECIA2

Plasmid

#115122

Purpose

Bait vector AT3G47090 L5_pECIA2 should be used with prey vector AT3G47090 L5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47090 (AT3G47090 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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XPS1 L6_pECIA2

Plasmid

#115123

Purpose

Bait vector XPS1 L6_pECIA2 should be used with prey vector XPS1 L6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47110 (AT3G47110 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT3G47570 L7_pECIA2

Plasmid

#115124

Purpose

Bait vector AT3G47570 L7_pECIA2 should be used with prey vector AT3G47570 L7_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47570 (AT3G47570 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT3G47580 L8_pECIA2

Plasmid

#115125

Purpose

Bait vector AT3G47580 L8_pECIA2 should be used with prey vector AT3G47580 L8_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G47580 (AT3G47580 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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ERL1 C11_pECIA2

Plasmid

#115129

Purpose

Bait vector ERL1 C11_pECIA2 should be used with prey vector ERL1 C11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G62230

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G16250 K9_pECIA2

Plasmid

#115132

Purpose

Bait vector AT2G16250 K9_pECIA2 should be used with prey vector AT2G16250 K9_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G16250 (AT2G16250 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT4G39270 K12_pECIA2

Plasmid

#115133

Purpose

Bait vector AT4G39270 K12_pECIA2 should be used with prey vector AT4G39270 K12_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39270 (AT4G39270 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge