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We narrowed to 3,970 results for: biorxiv

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Showing: 3581 - 3600 of 3970 results

  6. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid
    #223166
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Publication
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223166-plasmid-map-sequence-id-442868

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  9. pToxEXPr6

    Plasmid
    #218290
    Purpose
    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3
    Depositor
    Claudia Vickers
    Publication
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    Expression
    Yeast
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218290-plasmid-map-sequence-id-433735

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  10. pJQR3

    Plasmid
    #218289
    Purpose
    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6
    Depositor
    Claudia Vickers
    Publication
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W
    Expression
    Yeast
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218289-plasmid-map-sequence-id-433691

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  12. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid
    #216112
    Purpose
    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.
    Depositor
    Andre Berndt
    Publication
    Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    oROS-G_LF(C199S)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216112-plasmid-map-sequence-id-434850

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  13. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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  14. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  16. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  17. PASTA-2_trunc-pUC19_INT-Bxb1-attV_P2A_CD19CAR_bGH-pA

    Plasmid
    #247038
    Purpose
    Integration-Plasmid with Bxb1-attV (docking site) for bxb1-mediated integration of a CD19-CAR. Targeted whole plasmid insertion after maxiprep + AMPure bead purification.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Bxb1 phage attachment site (attP) = vector attachment site (attV), CD19-CAR
    Expression
    Mammalian
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247038-plasmid-map-sequence-id-495636

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  18. PASTA-5_pUC19_IVT-ePa01

    Plasmid
    #247041
    Purpose
    Plasmid for in vitro transcription (IVT) of enhanced Pa01 (ePa01) mRNA. Use indicated PCR-primers for correction of T7-mutation and addition of poly-A-tail.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    ePa01 recombinase (large serine integrase, LSR) for IVT
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247041-plasmid-map-sequence-id-500291

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  19. PASTA-1_pUC19_HDR-Bxb1-attG_TRAC_for-Cas12a

    Plasmid
    #247037
    Purpose
    Encodes HDR-template for Cas12a-mediated Knock-In of Bxb1-attG (landing pad) into TRAC gene (exon1). Template region to be PCR-amplified.
    Depositor
    Dimitrios Wagner
    Publication
    Kassing et al bioRxiv 2025.09.10.675267
    Insert
    Bxb1 bacterial attachment site (attB) = genomic attachment site (attG), flanked by human TRAC homology arms
    Expression
    Bacterial
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    247037-plasmid-map-sequence-id-499187

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  20. pHR_TRE-UAS_Gss-55SR4-LaM4_PGK_H2B-miRFP703

    Plasmid
    #259153
    Purpose
    Dual-input inducible expression of a secreted anti-mCherry nanobody dimer for inhibition of mCherry-mediated synNotch signalling. A constitutive fluorescent reporter is linked for sorting cells.
    Depositor
    Leonardo Morsut
    Publication
    Swedlund et al bioRxiv [Preprint]. 2026 May 25:2026.05.
    Insert
    anti-mCherry-PNE
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    TRE-UAS
    Available since
    July 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    259153-plasmid-map-sequence-id-516316

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Showing: 3581 - 3600 of 3970 results