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pCT-CMV-UBE3B-copGFP-Puro

Plasmid

#107342

Purpose

Expresses UBE3B fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

Ubiquitin-protein ligase E3B (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP(iL)-Grx1(human)

Plasmid

#65002

Purpose

Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP(iL)

Expression

Yeast

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Tfap2c

Plasmid

#128826

Purpose

dox-dependent expression of mouse Tfap2c

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tfap2c (Tfap2c Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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DNAJC11-EGFP

Plasmid

#210368

Purpose

Expresses DNAJC11 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NSP14-Flag

Plasmid

#210346

Purpose

Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pACE-K2P10.1-StrepTagII

Plasmid

#191475

Purpose

Expresses K2P10.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P10.1 (KCNK10, TREK-2 Human)

Expression

Insect

Mutation

N144Q (uniprot P57789-1)

Promoter

polH

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-PAFAH1B1_R22-

Plasmid

#92431

Purpose

mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Expression

Mammalian

Mutation

R22 (stop codon in position 22)

Promoter

CMV

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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(p)odr-3::GFP::EGL-4

Plasmid

#100897

Purpose

GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

Depositor

Noelle L'Etoile

Article

Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

Insert

56-2741: odr-3 promoter

Expression

Worm

Promoter

odr-3

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag

Plasmid

#154970

Purpose

Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

insert codes for EBA175 residues 1284 to 1462

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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HA-VHL C162F-pRc/CMV

Plasmid

#22042

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 162 from Cysteine to …

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMB71

Plasmid

#73085

Purpose

Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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HA-VHL 1-167-pRc/CMV

Plasmid

#22078

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-167 of wt VHL …

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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HA-VHL L158S-pRc/CMV

Plasmid

#22041

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant converting aa 162 from Cysteine to P…

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMB37

Plasmid

#73084

Purpose

Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR3c GW CHD5

Plasmid

#68869

Purpose

GW entry vector for CHD5

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Entry vector

Promoter

none

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-B-pCR

Plasmid

#46369

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

calretinin promoter (CALB2 Human)

Use

Luciferase

Tags

luciferase

Mutation

promoter

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3BΔHECT-copGFP-Puro

Plasmid

#107343

Purpose

Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(ΔHECT)-copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

deletion of HECT domain (757-end)

Promoter

CMV

Available Since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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