We narrowed to 7,247 results for: CIT

1. pAAV-EF1α1.1-GFP

   Plasmid

   #122100

   Purpose

   AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   GFP
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   May 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCerulean3-Bcl-W-FUGW

   Plasmid

   #166751

   Purpose

   Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bcl-W (Bcl2l2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   human ubiquitin C

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

   Plasmid

   #160623

   Purpose

   TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:dCas9:VPR-Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMG059 MBP-CREB-His

   Plasmid

   #154076

   Purpose

   Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CREB1 (CREB1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mCherry-FKBP-GEF(ITSN1)

   Plasmid

   #222631

   Purpose

   Cdc42 activation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PARP1_D770A-mCherry

   Plasmid

   #192260

   Purpose

   Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_D770A (PARP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D770A

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PARP1_R878A-mCherry

   Plasmid

   #192261

   Purpose

   Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_R878A (PARP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R878A

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-LYCHOS (GPR155) WT-HA

   Plasmid

   #199660

   Purpose

   Transient expression of LYCHOS (GPR155) WT

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ai95(RCL-GCaMP6f) targeting vector

   Plasmid

   #61579

   Purpose

   Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   CAG-LSL-GCaMP6-fast
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-Kss1

    Plasmid

    #52682

    Purpose

    Expression of Kss1 in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    KSS1 (KSS1 Budding Yeast)
    

    Use

    Tags

    His6 and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

    Plasmid

    #160568

    Purpose

    tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    M1_1-pTRNA-scf 2.1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EGFP.T2A.NCLX

    Plasmid

    #181873

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    

    Use

    AAV and Adenoviral

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    synthetic hybrid CAG promoter and synthetic hybri…

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE4889

    Plasmid

    #88905

    Purpose

    Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(D917A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 416Gal-SV40NLS-FUS-YFP

    Plasmid

    #29595

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    NLS-FUS (FUS Human)
    

    Use

    Tags

    SV40 NLS and YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pGEX6P2-MDMX (1-120)

    Plasmid

    #62065

    Purpose

    Expression of human MDM2 (1-120) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 1-120

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pHH0103_DST-1/1

    Plasmid

    #91288

    Purpose

    Protein expression and purification of human SH3 domain construct DST-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DST-1/1 (DST Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 426Gal-FUS-RRM Mutant-YFP

    Plasmid

    #29608

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS RRM mutant (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    F305L, F341L, F359L and F368L

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. mcry1K31R HA tag

    Plasmid

    #110297

    Purpose

    mammalian expression of full length mouse CRY1 mutant K31R

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mcry1 K31R (Cry1 Mouse)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    31 Lysines are changed to Argenine

    Promoter

    CMV

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-Bik-4E-pEGFP-C1

    Plasmid

    #166744

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Bik-4E (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRGEN-CMV-VPR-WT CjCas9 V-WT

    Plasmid

    #169914

    Purpose

    Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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