We narrowed to 13,531 results for: SUB;

1. pGEX-2T SHP1 deltaP

   Plasmid

   #8596

   Depositor

   Ben Neel

   Article

   Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

   Insert

   SHP1 (PTPN6 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deltaP (deletion of phosphatase domain, amino aci…

   Available Since

   June 21, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pGBW-m4046952

   Plasmid

   #145718

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046863

   Plasmid

   #145682

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046938

   Plasmid

   #145599

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pILGFP86

   Plasmid

   #83543

   Purpose

   Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   RPL4A promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   RPL4A

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK526

   Plasmid

   #155387

   Purpose

   PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   PphlF-TA4 in dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet RagA-RagC(L91P)

   Plasmid

   #99647

   Purpose

   Bacterial expression of RagA-RagC(L91P)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L91P

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-SAC1-HLx8

   Plasmid

   #108139

   Purpose

   SAC1 with extended linker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
   

   Expression

   Mammalian

   Mutation

   SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

   Promoter

   CMV

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUHD 10.3-human MIZ1 delta POZ

   Plasmid

   #74160

   Purpose

   Tet inducible expression of MIZ1 delta POZ

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MIZ1 delta POZ (ZBTB17 Human)
   

   Expression

   Mammalian

   Mutation

   delta POZ domain (first 109 aa)

   Promoter

   CMV2

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. GST-PRMT9 L182A/D183A/I184A/G185A

    Plasmid

    #67608

    Purpose

    bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    catalytic mutant LDIG(182-185)AAAA

    Promoter

    tac

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pBAD/HisB-PAiRFP2

    Plasmid

    #44271

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

    Insert

    PAiRFP2
    

    Expression

    Bacterial

    Mutation

    K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

    Promoter

    pBAD

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6_Ptrc_BsMdh_BmHps_BmPhi

    Plasmid

    #129104

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Expression

    Bacterial

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTJK512

    Plasmid

    #121469

    Purpose

    CEN LEU2 CNB1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Promoter

    Endogenous genomic promoter region

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. YFP(159-238) in pcDNAI/Amp

    Plasmid

    #60889

    Purpose

    This vector attaches YFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(159-238)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    SSA1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. QuasAr-P2A-GCaMP

    Plasmid

    #154082

    Purpose

    Independent expression of QuasAr and GCaMP under human synapsin 1 promoter

    Depositor

    Michael Hoppa

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

    Insert

    QuasAr-P2A-GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 327 to Alanine

    Promoter

    human synapsin 1

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-SERTG484E-myc

    Plasmid

    #107471

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    G484E

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-SERTS477F-myc

    Plasmid

    #107470

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expression

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    S477F

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4046831

    Plasmid

    #145671

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge