We narrowed to 4,572 results for: erf

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pHAGE TRE dCas9-KRAB

Plasmid

#50917

Purpose

Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

TRE

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Promoter

Ptet

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP1356

Plasmid

#119276

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hpGRISZ-TM

Plasmid

#248028

Purpose

Plasmid for packing AAV for hpGRISZ (synaptic zinc sensor)

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2026 Mar 3. doi: 10.1021/acsse

Insert

hpGRISZ

Use

AAV

Promoter

hSyn

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pminiTol2-5xUAS-hsp-sfGFP-zP2A-tetoff-zN2cG-zP2A-zTVA (UGNT-A)

Plasmid

#240272

Purpose

Helper plasmid for zebrafish rabies virus based-circuit tracing

Depositor

Xufei Du

Article

Chen et al eLife 13:RP100880

Inserts

N2cG (codon-optimized for Danio rerio)
sfGFP (codon-optimized for Danio rerio)
TVA (codon-optimized for Danio rerio)
tetoff (codon-optimized for Danio rerio)

Use

Zebrafish expression

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-CD5-NbHA-sfGFP

Plasmid

#242818

Purpose

Stable expression of NbHA-superfolderGFP for secretion of fusion protein into the extracellular medium

Depositor

Marvin Tanenbaum

Article

Rabouw et al Cell Systems, 2026; 17

Insert

NbHA

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

SFFV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCRISPRi_Mxi1_yl

Plasmid

#91248

Purpose

CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

Inserts

Codon optimized dCas9-Mxi1
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1360

Plasmid

#119278

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Expression

Mammalian

Promoter

CBH

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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Burden Monitor phi80 version

Plasmid

#66074

Purpose

The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP1337

Plasmid

#119270

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1358

Plasmid

#119277

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTSAR3.5t

Plasmid

#210257

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

eBFP2
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pdCRISPomyces-2BD

Plasmid

#234429

Purpose

Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

Depositor

Min-Kyu Oh

Article

Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

Insert

Modified dCas9(D10A, H840A) with polyaspartate linking

Expression

Bacterial

Mutation

Linked polyaspartate using glycine-serine linker

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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