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pLL1 sgRNA(MS2)-MS2-AIDmono

Plasmid

#112127

Purpose

empty sgRNA cloning vector with MS2-AIDmono

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono mutant (AICDA Human)

Use

CRISPR

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, H130A/R131E…

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBoBi-hLAMP2-C-PEN2

Plasmid

#183646

Purpose

Lentiviral expression of a lysosome-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and LAMP2 (PSENEN, LAMP2 Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hLAMP2-C-PEN2

Plasmid

#183646

Purpose

Lentiviral expression of a lysosome-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and LAMP2 (PSENEN, LAMP2 Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBoBi-hTOMM20-C-PEN2

Plasmid

#183647

Purpose

Lentiviral expression of a mitochondrion-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and TOMM20 (PSENEN, TOMM20 Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBoBi-hTOMM20-C-PEN2

Plasmid

#183647

Purpose

Lentiviral expression of a mitochondrion-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and TOMM20 (PSENEN, TOMM20 Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3 sgRNA(MS2)-MS2-AID3f

Plasmid

#112129

Purpose

empty sgRNA cloning vector with MS2-AID3f

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AIDmono 3f mutant (AICDA Human)

Use

CRISPR

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3 sgRNA(MS2)-MS2-AID3f

Plasmid

#112129

Purpose

empty sgRNA cloning vector with MS2-AID3f

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AIDmono 3f mutant (AICDA Human)

Use

CRISPR

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL2 sgRNA(MS2)-MS2-AID3c

Plasmid

#112128

Purpose

empty sgRNA cloning vector with MS2-AID3c

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono 3c mutant (AICDA Human)

Use

CRISPR

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL2 sgRNA(MS2)-MS2-AID3c

Plasmid

#112128

Purpose

empty sgRNA cloning vector with MS2-AID3c

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono 3c mutant (AICDA Human)

Use

CRISPR

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2-C17

Plasmid

#206385

Purpose

Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2-C17 chimera (SOX2, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2-C17

Plasmid

#206385

Purpose

Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2-C17 chimera (SOX2, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3-hAP1 1-419

Plasmid

#183644

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only amino aci…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3-hAP1 1-419

Plasmid

#183644

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only amino aci…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBoBi-hAP1 Δ420-440

Plasmid

#183642

Purpose

To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Use

Lentiviral

Tags

HA

Mutation

truncated ATP6AP1 mutant lacking the transmembran…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBoBi-hAP1 Δ420-440

Plasmid

#183642

Purpose

To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Use

Lentiviral

Tags

HA

Mutation

truncated ATP6AP1 mutant lacking the transmembran…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MG1655 ΔendA ΔrecA (DE3)

Bacterial Strain

#37854

Depositor

Kristala Prather

Article

Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

Bacterial Resistance

None

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only