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pJBL708

Plasmid

#140378

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 10BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-10BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL709

Plasmid

#140379

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 12BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-12BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJB03

Plasmid

#111961

Purpose

Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

Tac

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1400

Plasmid

#78190

Purpose

Ce hsp-16.1 expression in worms

Depositor

Junho Lee

Article

Lee et al J Biol Chem. 2013 Jan 18;288(3):1582-9.

Insert

hsp-16.1 (hsp-16.1 Nematode)

Expression

Worm

Promoter

hsp-16.1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pCRII HSPB8

Plasmid

#61035

Purpose

Probe for HSPB8 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB8 (hspb8 Zebrafish)

Expression

Bacterial

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM29i2

Plasmid

#59607

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM7i2

Plasmid

#59603

Purpose

Expression of shRNA against human ADAM7

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM7 (ADAM7 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-DRAM1i2

Plasmid

#59597

Purpose

Expression of shRNA against human DRAM1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

DRAM1 (DRAM1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-DRAM1i1

Plasmid

#59596

Purpose

Expression of shRNA against human DRAM1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

DRAM1 (DRAM1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
TAL3085

Plasmid

#41259

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ghsra-Right (ghsra Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3084

Plasmid

#41258

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ghsra-Left (ghsra Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00289

Plasmid

#34415

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12942 (CG12942 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
hPrp16 C-terminus

Plasmid

#11205

Depositor

Robin Reed

Article

Zhou et al EMBO J. 1998 Apr 1. 17(7):2095-106.

Insert

Prp16 C-terminus (DHX38 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 948-1227

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSL1765 (pSPIN-R, R*MmeI)

Plasmid

#160734

Purpose

Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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wt Opa1

Plasmid

#204427

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Control-1

Plasmid

#204442

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L243A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Pa01 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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[#GS16-LV] Syn1-MitoTRACER - Donor

Plasmid

#233565

Purpose

Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Lentiviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo

Plasmid

#239948

Purpose

Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD6(L3)

Expression

Bacterial and Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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