We narrowed to 6,005 results for: 338

1. EF1a_KLF10_P2A_Hygro_Barcode

   Plasmid

   #120496

   Purpose

   Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF10 (KLF10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMX-IY HA-GBRPL1

   Plasmid

   #89297

   Purpose

   For expression of the mammalian Atg8 protein GABARAPL1 tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Retroviral

   Tags

   HA and IRES-YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flrt1-Fc-His

   Plasmid

   #72073

   Purpose

   Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Flrt1 (Flrt1 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2 mCherry-Rtn4HD

   Plasmid

   #86685

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4 homology domain (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   homology domain only

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBMN mCherry-p62(W340A/ΔUBD)

   Plasmid

   #119688

   Purpose

   For mammalian expression of LIR mutant p62 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   p62 (SQSTM1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W340A & UBD deletion (1-385)

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-myc-EphB1

   Plasmid

   #200981

   Purpose

   Mammalian expression plasmid for myc-tagged EphB1

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB1 (EPHB1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR3-FLAG-Gephyrin GC

   Plasmid

   #68818

   Purpose

   Flag tagged mammalian expression of GC domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2 GFP-Rtn4HD

   Plasmid

   #86686

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4 homology domain (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   homology domain only

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-HypaCas9-mR2-RHOA_sgRNA

   Plasmid

   #183877

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_MAP3K9-1/2

    Plasmid

    #91313

    Purpose

    Protein expression and purification of human SH3 domain construct MAP3K9-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MAP3K9-1/2 (MAP3K9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_ARHGEF9-1/1

    Plasmid

    #91417

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF9-1/1 (ARHGEF9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX/PD-L1 3SA

    Plasmid

    #121467

    Purpose

    Expression of GST-human PD-L1 in E. Coli

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S176A, T180A, S184A

    Promoter

    Tac

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMKO.1-shCcnb1

    Plasmid

    #162793

    Purpose

    Ccnb1 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Ccnb1 shRNA (Ccnb1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-NLS-gs10-cDHFR174

    Plasmid

    #188256

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cDHFR174 (DHFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HtrA2-FLAG S400D

    Plasmid

    #15940

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S400D

    Promoter

    Available since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. FCGR2a-bio-His

    Plasmid

    #51754

    Purpose

    Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FCGR2a (FCGR2A Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Ctdnep1_D67E-2xFlag

    Plasmid

    #196519

    Purpose

    Expression of Ctdnep1_D67E-2xFlag

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead)

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+CeGFP-Ctdnep1WTsiR

    Plasmid

    #196517

    Purpose

    Expression of Ctdnep1WT-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1WTsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    CMV promoter; T7 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. [UBC][EIF4e][GG cloning]

    Plasmid

    #97189

    Purpose

    EIF4e plasmid with Pum cloning site

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    EIF4E variant 1 (EIF4E Human)
    

    Use

    Synthetic Biology

    Tags

    empty Pum cloning site

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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