We narrowed to 38,917 results for: LAT;

1. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84242

   Purpose

   Expresses GA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBM38

   Plasmid

   #155899

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   RBM38 (RBM38 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-V5-PTPN14-PPxY

   Plasmid

   #61006

   Purpose

   Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 PPxY mutation (PTPN14 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   PPxY mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 T7 DLC1

   Plasmid

   #24266

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   DLC1 (DYNLL1, DLC1 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pBabe EGFR ASVinsertion

   Plasmid

   #32066

   Depositor

   Matthew Meyerson

   Article

   Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Insert duplicate A767-V769

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-EZH2-T487A mutant

   Plasmid

   #28061

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T487A

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA3-V5-PTPN14-del-N

   Plasmid

   #61004

   Purpose

   Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 delete N (PTPN14 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 17-310

   Promoter

   CMV

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

8. MERS-coV-NSP3-c001

   Plasmid

   #173085

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   D1109-D1275, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIS1-Vim/Actb5UTR-renilla

   Plasmid

   #38238

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Actb promoter (1000 nt upstream) and 5' UTR …

   Promoter

   Endogenous Vim

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3-V5-PTPN14-del-C

    Plasmid

    #61005

    Purpose

    Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 delete C (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 934-1187

    Promoter

    CMV

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBabe EGFR Del4

    Plasmid

    #32064

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Delete L747-P753, insert S

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pBabe EGFR(G719S/T790M)

    Plasmid

    #32076

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G719S/T790M

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pBabe EGFR Del5

    Plasmid

    #32063

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Delete E746-S752, insert V)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pJZC25

    Plasmid

    #62328

    Purpose

    sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

    Plasmid

    #214877

    Purpose

    Lentiviral vector encoding RfxCas13d targeting LPT guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crLPT-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-SOCS6-WT

    Plasmid

    #74496

    Purpose

    Overexpression of SOCS6 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    SOCS6 (Socs6 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pBABE-Flag-Cdk9-T186A-IRES-eGFP

    Plasmid

    #28097

    Depositor

    Andrew Rice

    Article

    Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.2-Cx43-siResist

    Plasmid

    #49852

    Purpose

    Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

19. F11-R pEBio

    Plasmid

    #61486

    Purpose

    Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F11R (F11R Human)
    

    Use

    Tags

    enzymatic biotinylation sequence and rat CD4 d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. PPIB

    Plasmid

    #155838

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPIB (PPIB Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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