We narrowed to 29,617 results for: ple

1. pAAV-hsyn-FLPo-T2A-GFP

   Plasmid

   #161766

   Purpose

   express FLPo and GFP under the control of human synapsin promoter

   Depositor

   Baoji Xu

   Article

   An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

   Inserts

   FLPo
   T2AEGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI118-SiT-Cas12a-[Ind]

   Plasmid

   #128406

   Purpose

   Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_TRE_Z18-PDGFR_EF1a_eGFP

   Plasmid

   #186304

   Purpose

   Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z18-PDGFR
   eGFP
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-HpaBC

   Plasmid

   #71260

   Purpose

   Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

   Depositor

   Mattheos Koffas

   Article

   Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

   Inserts

   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP L202 gRNA

   Plasmid

   #119132

   Purpose

   gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   eGFP L202 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   U6

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCl-MCS_miniCMV_miRFP703

   Plasmid

   #134985

   Purpose

   The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   miRFP703
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF6 VPS52-V5-6His

   Plasmid

   #175096

   Purpose

   V5 tagged VPS52 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

   Insert

   VPS52 (VPS52 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   N666D- please see depositor comment

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI108-SiT-Cas12a-[Cond]

   Plasmid

   #128407

   Purpose

   Conditionally express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRG706_lexO-SbfI_LexA-TF_LEU2MX

   Plasmid

   #154818

   Purpose

   Inducible expression of SbfI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   SbfI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG2abc

    Plasmid

    #80617

    Purpose

    pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG2abcd

    Plasmid

    #80618

    Purpose

    pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pBIG1c

    Plasmid

    #80613

    Purpose

    pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG1d

    Plasmid

    #80614

    Purpose

    pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBIG1e

    Plasmid

    #80615

    Purpose

    pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-ICUE3-NLS

    Plasmid

    #138218

    Purpose

    Nuclear-targeted FRET-based cAMP indicator.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    ICUE3-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-PLIN2-tdTOMATO

    Plasmid

    #172533

    Purpose

    PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F, and R415H mutations in …

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRG659_lexO-FseI_LexA-TF_LEU2MX

    Plasmid

    #154816

    Purpose

    Inducible expression of FseI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    FseI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBbi-TIR1-tTA/Pur

    Plasmid

    #171683

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Inserts

    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter and RPBSA promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRG646_lexO-HO_LexA-TF_LEU2MX

    Plasmid

    #154815

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge