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pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

Plasmid

#63170

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-OLLAS"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Myc/TSSK1

Plasmid

#199624

Purpose

Expression vector of mouse TSSK1. Myc-tag at N-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tssk1 mRNA (Tssk1 Mouse)

Tags

Myc-tag

Promoter

CAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY1026 COX8aN::miniSOG in pENTR[1-2]

Plasmid

#66752

Purpose

miniSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

COx8aN (N terminal 29 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BKRF3

Plasmid

#195790

Purpose

Gateway Entry Clone of Epstein-Barr virus BKRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BKRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF2

Plasmid

#195800

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-MAVS-5'UTR

Plasmid

#184639

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pBSSK p85 alpha (delta SH3)

Plasmid

#13430

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta SH3) (PIK3R1 Human)

Tags

FLAG

Expression

Bacterial

Mutation

SH3 domain replaced by flag tag.

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
RNAseq_cal_Tuschl

Plasmid

#59426

Purpose

Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

Depositor

Thomas Tuschl

Article

Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

Insert

RNAseq_cal_Tuschl

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a+GFP-CAMSAP1 CKK

Plasmid

#59031

Purpose

expresses CKK domain of CAMSAP1 in E. coli

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 CKK domain (CAMSAP1 Human)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-NIrD-Mefv iso1

Plasmid

#92108

Purpose

inducible expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L_R986C

Plasmid

#206176

Purpose

mammalian expression of SAMD9L R986C

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Tags

GFP

Expression

Mammalian

Mutation

R986C

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2a

Plasmid

#195767

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2a

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2a (BNLF2a Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2b

Plasmid

#195770

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2b

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2b (BNLF2b Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO-TVA-mCherry

Plasmid

#149295

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Mutation

Start codon removed.

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRRG304-Ndk

Plasmid

#99072

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ndk (nou darake)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ndk (nou darake) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6p-Meis1a

Plasmid

#78144

Purpose

bacterial expression of GST-Meis1a

Depositor

Francesco Blasi

Article

Dardaei et al Proc Natl Acad Sci U S A. 2014 Mar 11;11

Insert

Meis1a (Meis1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pBABE-hygro NM23 H1 WT

Plasmid

#11364

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pPRIME-TREX-GFP-FF3

Plasmid

#11667

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pBW300ParsR-Amp32T

Plasmid

#78652

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only