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We narrowed to 7,649 results for: Pol

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Showing: 3721 - 3740 of 7649 results

  10. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid
    #131006
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    V5 - 10 total of V5 epitope tag
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131006-plasmid-map-sequence-id-305181

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  13. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid
    #217804
    Purpose
    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-linker-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217804-plasmid-map-sequence-id-432897

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  14. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid
    #208946
    Purpose
    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208946-plasmid-map-sequence-id-415680

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  15. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid
    #193721
    Purpose
    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193721-plasmid-map-sequence-id-383728

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  16. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  17. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131007-plasmid-map-sequence-id-305185

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  18. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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  19. pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc

    Plasmid
    #196241
    Purpose
    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus
    Depositor
    Robert Sobol
    Article
    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.
    Insert
    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
    Tags
    GST tag and myc tag
    Expression
    Bacterial
    Mutation
    amino acid 35 lysine is replaced with glutamic ac…
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    196241-plasmid-map-sequence-id-398155

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Showing: 3721 - 3740 of 7649 results