We narrowed to 9,001 results for: ef1;

1. PCT62

   Plasmid

   #170391

   Purpose

   expresses three sgRNA cassettes

   Depositor

   Jonathan Weissman

   Article

   Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

   Insert

   PCT62-targetsite
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LES2A-CRE

   Plasmid

   #167933

   Purpose

   Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   mScarlet-P2A-CRE
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI-neo Flag HAUSP

   Plasmid

   #16655

   Depositor

   Bert Vogelstein

   Article

   Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

   Insert

   HAUSP (USP7 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG4

   Plasmid

   #162605

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. opto-RhoGEF-mCherry_pcDNA3.1

   Plasmid

   #164473

   Purpose

   Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

   Insert

   opto-RhoGEF
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG2

   Plasmid

   #162603

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGAMA-empty

   Plasmid

   #74755

   Purpose

   Mammalian lentiviral vector

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. vYM140_PB-pEFa1-iaaM(P.s)-Hygro

   Plasmid

   #160043

   Purpose

   Expressing iaaM for converting L-Trp to IAM

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   iaaM (iaaM )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVITRO1-mCardinal-CAXX

   Plasmid

   #184451

   Purpose

   Plasma membrane-targeted mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT3928

    Plasmid

    #113331

    Purpose

    control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVITRO1-TagRFP-NLS

    Plasmid

    #184446

    Purpose

    Nucleus-targeted TagRFP expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    TagRFP-3xNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas9

    Plasmid

    #162606

    Purpose

    Pre-express Cas9 in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKL07

    Plasmid

    #104431

    Purpose

    mitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    mito-Super Ecliptic pHluorin-mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-T3G-CERP2

    Plasmid

    #175498

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXPR_124

    Plasmid

    #133461

    Purpose

    EF1a promoter expresses SpyoCas9 and P2A provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    SpyoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PP244

    Plasmid

    #78978

    Purpose

    Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF221

    Plasmid

    #121669

    Purpose

    U6-sgRNA EF1a-mCherry

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-AP.Baz1a.1-GFP-WPRE

    Plasmid

    #211490

    Purpose

    expresses GFP under artificial promoter Baz1a

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RAB14 S25N

    Plasmid

    #61495

    Purpose

    to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S25N, inactive form

    Promoter

    EF1a

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only