163,253 results

1. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

   Plasmid

   #61665

   Purpose

   Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCA/GAL4(1-93).ER.VP16

   Plasmid

   #108216

   Purpose

   Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

   Depositor

   Didier Picard

   Article

   Louvion et al Gene 1993 Sep 6;131(1):129-34.

   Insert

   Gal4 DBD-ERalpha HBD-VP16
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERalpha HBD has G400V mutation

   Promoter

   ADH1

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVpuro-PCAF

   Plasmid

   #63705

   Purpose

   retroviral expression of human PCAF in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   PCAF (KAT2B Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   E452G

   Promoter

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EGFR-H773_V774insNPH

   Plasmid

   #116262

   Purpose

   Lentiviral expression of EGFR H773_V774insNPH

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   H773_V774insNPH

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP Sec22b

   Plasmid

   #101918

   Purpose

   Expresses Sec22b in mammalian cells

   Depositor

   Thierry Galli

   Article

   Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

   Insert

   Sec22b (Sec22b Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. aCKAR

   Plasmid

   #124427

   Purpose

   FRET-based reporter for monitoring aPKC activity in cells

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

   Insert

   aCKAR
   

   Use

   Tags

   mCFP and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSin Helper

   Plasmid

   #127694

   Purpose

   Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Glycoproteins
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

8. Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

   Plasmid

   #187100

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   mCherry-SL2

   Expression

   Worm

   Mutation

   Promoter

   Ttx-3

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide-Neo

   Plasmid

   #139449

   Purpose

   Lentiviral vector with gRNA scaffold and neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   no sgRNA inserted; resistance gene: neoR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC103-PBNeo-U6-sgRNA-Expression

    Plasmid

    #48228

    Purpose

    sgRNA expression on PiggyBac vector with Neo-selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hAur-A

    Plasmid

    #8510

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hAur-A (AURKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEX3*-SBP-GFP

    Plasmid

    #120174

    Purpose

    Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Peroxisomal biogenesis factor 3 (PEX3 Human)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 60-p3E_INTRON-EmptyRNAi

    Plasmid

    #163380

    Purpose

    p3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).

    Depositor

    Jean Giacomotto

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EBFP2-H2B-6

    Plasmid

    #55243

    Purpose

    Localization: Nucleus/Histones, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBUN6A11

    Plasmid

    #50579

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_FLI1_P2A_Hygro_Barcode

    Plasmid

    #120437

    Purpose

    Barcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEGFP-H4-23

    Plasmid

    #56463

    Purpose

    Localization: Nucleus/Histones, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    H4 (HIST1H4J Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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