We narrowed to 4,700 results for: golden gate

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pYPQ134B2.0

Plasmid

#167158

Purpose

Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pNP3620 [pNP2916 - ISCro4 bRNA (TBL2, WT + DBL1, WT)]

Plasmid

#247267

Purpose

Express ISCro4 bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2916 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pNP3396 [pNP2922 - ISCro4 DBL1 (WT) + ISCro4 Recombinase (WT)]

Plasmid

#247259

Purpose

Express ISCro4 recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2922 - ISCro4 bRNA112-179 (DBL1)

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNP568

Plasmid

#222725

Purpose

pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW91013

Plasmid

#222450

Purpose

Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::RUBY::OCS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW101008

Plasmid

#222453

Purpose

Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::OsL5::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ133B2.0

Plasmid

#99892

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ135B2.0

Plasmid

#167159

Purpose

Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pNP1047

Plasmid

#222726

Purpose

pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbV-EGFP

Plasmid

#216161

Purpose

Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Type

Empty backbone

Use

Lentiviral

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAGC-Lb12-P1Bb

Plasmid

#231387

Purpose

For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

Depositor

Qi-Jun Chen

Article

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

U6-29

Use

CRISPR

Expression

Plant

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX1-TET-VP16-ZF43-2A-tagBFP-bGH_DIVrtTA_pAMB282

Plasmid

#246379

Purpose

ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the TET promoter with rtTA expressed divergently

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

Plasmid

#181792

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pNP1954

Plasmid

#222730

Purpose

pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNP2270

Plasmid

#222733

Purpose

pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Helper (lacZ)

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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