We narrowed to 21,977 results for: Sis

1. pLV-mEGFP-hCTNNA1_DM1-domain

   Plasmid

   #234555

   Purpose

   CTNNA1 M1-domain deletion mutant

   Depositor

   Cara Gottardi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

   Insert

   mEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Deletion of aa 271-396

   Promoter

   CMV

   Available since

   June 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-AtmiR173aTS-B/c

   Plasmid

   #227964

   Purpose

   Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Inserts

   B/c
   AtmiR173aTS
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MT2

   Plasmid

   #223330

   Purpose

   Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
   

   Use

   Tags

   3HA-TurboID-EGFP

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-HA-CBS(opt)-FLAG

   Plasmid

   #230088

   Purpose

   expressing human CBS

   Depositor

   Xingguo Zhu

   Article

   Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

   Insert

   CBS (CBS Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Multiple wobble mutations

   Promoter

   CMV

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-HA-L2HGDH(opt)-FLAG

   Plasmid

   #230089

   Purpose

   expressing human L2HGDH

   Depositor

   Xingguo Zhu

   Article

   Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

   Insert

   L2HGDH (L2HGDH Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Multiple wobble mutations

   Promoter

   CMV

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MT3

   Plasmid

   #223331

   Purpose

   Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
   

   Use

   Tags

   3HA-EGFP-miniTurboID

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MT4

   Plasmid

   #223332

   Purpose

   Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
   

   Use

   Tags

   3HA-miniTurboID-EGFP

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSuper Retro GFP DGK zeta human

   Plasmid

   #224579

   Purpose

   To stably downregulate the expression of human DGK zeta in human cells

   Depositor

   Isabel Mérida

   Article

   Torres-Ayuso et al Oncogenesis. 2015 Aug 24;4(8):e164. doi:

   Insert

   sh RNAi Diacylglycerol kinase zeta human (GTPBP6 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1BR9-AtWUSSTOP-Δα3

   Plasmid

   #202056

   Purpose

   E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
   

   Use

   Tags

   6xHis and GFP11

   Expression

   Bacterial

   Mutation

   third helix of homeodomain deleted (AA82-102)

   Promoter

   T7 Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC_hfnCas12a_crRNA NR2

    Plasmid

    #176248

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNOC_hfnCas12a_crRNA NR1

    Plasmid

    #176247

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNOC_hfnCas12a_crRNA NR3

    Plasmid

    #176249

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid

    #153445

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid

    #153444

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAN19-TIR1-GUS-NOSt

    Plasmid

    #108547

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAN19-ccvTIR1-GUS-NOSt

    Plasmid

    #108548

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Changed F79 to G

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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