We narrowed to 4,489 results for: TIL;

1. pAAV-VTKD4-ChR2-YFP

   Plasmid

   #178721

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   ChR2-YFP
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-mVenus-PA-Rac1-T17N

   Plasmid

   #22017

   Purpose

   Expression of photoactivatable Rac1 (dominant-negative mutant)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
   

   Tags

   6X His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutation T17N.

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-TEV-BirA-FLAG

   Plasmid

   #105786

   Purpose

   Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-BirA-FLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-mRuby3-TEV

   Plasmid

   #105780

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mRuby3-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-TEV-MBP

   Plasmid

   #105787

   Purpose

   Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-MBP

   Expression

   Mammalian

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-TEV-mRuby3

   Plasmid

   #105797

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mRuby3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLP-PIGA2 (pLP300)

   Plasmid

   #168462

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mDrc7-FLAG

   Plasmid

   #176470

   Purpose

   Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   dynein regulatory complex subunit 7 (Drc7 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO-FUW-Mecom 1

   Plasmid

   #118590

   Purpose

   Expresses Mecom isoform 1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Mecom (Mecom Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. eA3A-BE3 (pRZ206)

    Plasmid

    #131315

    Purpose

    CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-TEV-mCherry

    Plasmid

    #105796

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid

    #136660

    Purpose

    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SITS-mCherry-Bin1b

    Plasmid

    #176025

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Triple ORF LoxP ifgMosaic Donor (LH208)

    Plasmid

    #99625

    Purpose

    To clone the 3 ORFs for Cre dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    N-PhiM
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MEF2A-Sensor

    Plasmid

    #208928

    Purpose

    in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    Mef2a docking sequence+Phospho site (MEF2A Human)
    

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

    Plasmid

    #22034

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450M (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450M.Rac1 s…

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pINDUCER20 MEF2C S222D

    Plasmid

    #107234

    Purpose

    MEF2C phosphomimetic mutant serine to aspartic acid

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LoxP1-His-H2B-Cherry-2A (SO290)

    Plasmid

    #99616

    Purpose

    To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    6xHis-H2B-Cherry
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-Kss1

    Plasmid

    #52682

    Purpose

    Expression of Kss1 in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    KSS1 (KSS1 Budding Yeast)
    

    Tags

    His6 and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

20. PLL5.0-Anillin ShRNA-GFP-AnillinFL

    Plasmid

    #187272

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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