We narrowed to 6,113 results for: arl

1. pLB052 - deAct - SpVB

   Plasmid

   #136286

   Purpose

   entry vector for GreenGate cloning method, D module, open reading frame of the mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvB

   Depositor

   Alexis Maizel

   Article

   Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

   Insert

   deAct - SpvB
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-TetO-Lin28

   Plasmid

   #60345

   Purpose

   Mammalian Expression, Lentiviral

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Lin28 (Lin28a Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Minimal CMV + TetO

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. YA1531: pAAV_CAG-FLEX-QuasAr3

   Plasmid

   #107701

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   QuasAr3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

   Plasmid

   #84244

   Purpose

   Expresses GA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sa dCas9
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL680 CHYRON3 integration construct

   Plasmid

   #126448

   Purpose

   To integrate the CHYRON3 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6/3xLacO-CHYRON3 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6/3xLacO

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pbG-APRT-J-u32uni-PdTKmCh

   Plasmid

   #107279

   Purpose

   APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   APRT (APRT Human)
   puro-deltaTK; CAG::mCherry
   

   Mutation

   c.402A>T, c.407T>C

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.E2.ChETA

   Plasmid

   #220310

   Purpose

   Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

   Depositor

   Matt Rowan

   Article

   Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

   Insert

   ChETA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   S5E2

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YA1629: pAAV_CAG-FLEX-paQuasAr3-s

   Plasmid

   #107703

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   paQuasAr3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

    Plasmid

    #107704

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3-P2A-CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)mGAT1YFP3

    Plasmid

    #41667

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 3 C-terminal-m…

    Promoter

    CMV

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJM186A

    Plasmid

    #218127

    Purpose

    Hybrid circuit displaying hsCRBN bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII
    

    Tags

    434cI_RR69

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123338

    Purpose

    Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    DAKAP-Venus-cpVenus-FLARE-AKAR
    

    Tags

    N-terminal 30 amino acids from DAKAP1, Venus, and…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. nes714tk/lacZ

    Plasmid

    #47614

    Purpose

    used to create transgenic mice expressing LacZ reporter with Nestin enhancer

    Depositor

    Urban Lendahl

    Article

    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

    Insert

    nestin 2nd intron fragment (NES Human)
    

    Use

    Enhancer reporter

    Tags

    HSV tk promoter and lacZ

    Mutation

    714 bp from 3' end of 2nd intron

    Promoter

    HSV tk promoter

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121814

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKI-cjFOXP2-hum

    Plasmid

    #129757

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    FOXP2 (LOC100411307 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Mutation

    T301N/N323S

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC8_PH-GST-Nter-GWs-Lox (VE5586)

    Plasmid

    #163766

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal GST tag
    

    Tags

    GST Tag

    Expression

    Insect

    Promoter

    PH

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid

    #121841

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge