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We narrowed to 18,824 results for: nls

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Showing: 3761 - 3780 of 18824 results

  5. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Publication
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129531-plasmid-map-sequence-id-255632

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  6. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Publication
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

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  8. deltaVP1 + VP2C-GFP-MIOX

    Plasmid
    #166682
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166682-plasmid-map-sequence-id-329930

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  9. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159861
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Publication
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159861-plasmid-map-sequence-id-318036

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  10. vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

    Plasmid
    #160044
    Purpose
    For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)
    Depositor
    Michael Elowitz
    Publication
    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iaaH (iaaH )
    Use
    Synthetic Biology
    Tags
    3xNLS-Citrine
    Expression
    Mammalian
    Promoter
    pEF1s
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    160044-plasmid-map-sequence-id-318469

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  17. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  18. Dendra2-PCNA-19-SV40NLS-4

    Plasmid
    #57735
    Purpose
    Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. mTFP1-PCNA-19-SV40NLS-4

    Plasmid
    #55505
    Purpose
    Localization: Nucleus, Excitation: 462, Emission: 492
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mTFP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139086-plasmid-map-sequence-id-273882

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Showing: 3761 - 3780 of 18824 results