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Plasmid#110734PurposeRecombination template for replacing CDKN2A Exon2 with CMV-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseOtherAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMonAID_dCas9-PmCDA_Hyg_ALS
Plasmid#91692PurposeMonocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A and H840A for dead Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PttrB185-269Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) TFRC
Plasmid#74709PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
UseCRISPRExpressionMammalianPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Tubulin-6
Plasmid#57431PurposeLocalization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTubulin (TUBA1B Human)
TagsmEos2ExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
mCherry-EB1-C-18
Plasmid#55036PurposeLocalization: MT End Binding Protein, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB1 (MAPRE1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1364 LV EF1a-CD4 IRES-EGFP
Plasmid#201920Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD4DepositorAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN7CAST
Plasmid#190661PurposeExpresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
ExpressionMammalianPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 KRAB
Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…Available sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_mCherry
Plasmid#182939Purposealpha7 nACHR expression in mammalian cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNACH7 (CHRNA7 Human)
UseSynthesizedExpressionMammalianPromoterEF1alphaAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA2 TKIT donor
Plasmid#169421PurposeDonor DNA for TKIT knockin of SEP to GluA2 in mouseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSuper ecliptic pHluorin (SEP)
UseCRISPRExpressionMammalianAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
hyA3A-BE4max
Plasmid#157943PurposeMammalian expression of hyA3A-BE4maxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthyA3A-BE4max
UseCRISPRExpressionMammalianAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp10
Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
OSCA1.2
Plasmid#136593PurposeExpresses the mechanosensitive channel OSCA1.2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOSCA1.2 (AT4G22120 Mustard Weed)
TagspIRES2-mCherryExpressionMammalianPromoterCMVAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-avGFP
Plasmid#129508PurposeExpresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)DepositorInsertavGFP
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXGFPC-5 Pxyl-dcas9 (Sth3)
Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedPromoterxyloseAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-N2cP
Plasmid#100808Purposeexpress rabies virus CVS N2c strain phosphoprotein gene (N2cP)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrabies virus CVS N2c strain phosphoprotein gene
ExpressionMammalianPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by