We narrowed to 2,727 results for: BFP

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pUFlip-floxed2A-mRFP-; HE1A:BFP -1

Plasmid

#180008

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -1

Plasmid

#173887

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSpCas9_T2A_mCherry sg1-sg2

Plasmid

#192479

Purpose

All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

Plasmid

#128607

Purpose

Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-EF1a-BbTagBY

Plasmid

#45185

Depositor

Dawen Cai, Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has service

AAV9

Insert

pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Publication

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

Plasmid

#140086

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

Depositor

Stanley Qi

Publication

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro-T2A-BFP

Expression

Mammalian

Promoter

U6

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Plasmid

#233444

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-MCP

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

Plasmid

#173891

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

Plasmid

#213119

Purpose

Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

Depositor

Scott Coyle

Publication

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Publication

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Promoter

lac

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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CTp132-Reporter-T

Plasmid

#256441

Purpose

Mammalian expression of a TSM with half of GFP-2A-BFP with best targeting spacer

Depositor

Patrick Hsu

Publication

Chandrasekaran et al Cell Syst. 2026 Feb 18;17(2):101487. doi

Insert

Trans-splicing module with half of GFP-2A-BFP with best targeting spacer

Expression

Mammalian

Promoter

SFFV

Available since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

Plasmid

#233443

Purpose

PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Plasmid

#233446

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-PCP

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Plasmid

#233442

Purpose

PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

Plasmid

#233441

Purpose

PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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CIDAR MoClo Parts Kit

Plasmid kit

#1000000059

Purpose

Collection of standardized parts for combinatorial design and expression tuning in E. coli.

Depositor

Douglas Densmore

Application

Cloning and Synthetic Biology

Vector type

Bacterial Expression

Cloning type

Golden Gate (MoClo)

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only