We narrowed to 2,395 results for: EFS

1. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235319

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

   Plasmid

   #235304

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   FXN-P2A-mRuby2 (FXN Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235322

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235324

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

   Plasmid

   #235305

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   FXN-P2A-mRuby2 (FXN Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G (3rd-generation Tet system)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5FRT-EF-Pdgfralpha-EGFPN

   Plasmid

   #66787

   Purpose

   Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2+-FLAG3-drAgo2DY-EF

   Plasmid

   #221716

   Purpose

   Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2DY-EF (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   D651E and Y680F in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PT7lacO-EF-Ts-His6

   Plasmid

   #224371

   Purpose

   Express EF-Ts protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-Ts (tsf E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PT7lacO-EF-G-His8

   Plasmid

   #224369

   Purpose

   Express EF-G protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-G (fusA E. coli)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PT7lacO-EF-Tu-His8

    Plasmid

    #224370

    Purpose

    Express EF-Tu protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    EF-Tu (tufB E. coli)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

    Plasmid

    #66790

    Purpose

    Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRbeta (Pdgfrb Mouse)
    

    Use

    Tags

    C-term EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

    Plasmid

    #109995

    Purpose

    Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. px335-EF-Slc2a3-L

    Plasmid

    #122304

    Purpose

    Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

16. px335-EF-Slc2a3-R

    Plasmid

    #122305

    Purpose

    Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEGFP-EF(N)-30nmSAH

    Plasmid

    #61679

    Purpose

    GFP-labeled SAH scaffold with EF Hand attachment site

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    mEGFP-EF(N)-30nmSAH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG3-drAgo2DY-EF

    Plasmid

    #221733

    Purpose

    Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2DY-EF (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E and Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTR(EF)-tRNA(Q)

    Plasmid

    #170517

    Purpose

    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(EF)-tRNA(Q)
    

    Use

    Pcr template vector for amplifying grnacore(ef)-t…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLR5-U6-sgRNA-EF

    Plasmid

    #82635

    Purpose

    piggyBac vector for expressing modified sgRNA in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Type

    Empty backbone

    Use

    CRISPR; Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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