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We narrowed to 413 results for: PEF1

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pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

Plasmid

#225699

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187334

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT259 Lenti pEF-dCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast

Plasmid

#187335

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Cas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225703

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

Plasmid

#195548

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

Plasmid

#195545

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
gTBEv4

Plasmid

#220620

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Use

Tags

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187337

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

Plasmid

#195549

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

Plasmid

#195550

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

Plasmid

#225698

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

Plasmid

#188758

Purpose

Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

VP64

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

Plasmid

#195547

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPL001

Plasmid

#184750

Purpose

Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

pEF1a-eGFP-T2A-BSD-bGHpA

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT350 Lenti pEF-dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

Plasmid

#187347

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT351 Lenti pEF-dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast

Plasmid

#187348

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Plasmid

#187332

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187333

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge