-
Plasmid#123698PurposeEncodes pUAS (miniSV40 core) as a Type 2 part to be used in the MTK systemDepositorInsertpUAS (mini SV40)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-Omicron-BA.5
Plasmid#213828PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variantDepositorInsertpαH-S-GSAS/Omicron-2P-BA5 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR2
Plasmid#167001PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 2 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-AMP-Bv16SrRNA
Plasmid#218518PurposepUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantificationDepositorInsert16S rRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-AMP-Fp16SrRNA
Plasmid#218519PurposepUCIDT-AMP containing 16S rRNA gene of F. prausnitzii for absolute concentration quantificationDepositorInsert16S rRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDGFR-TMD (MTK 3B) (pZYW025)
Plasmid#194218PurposeMammalian Toolkit part 3b encoding the transmembrane domain of PDGFRDepositorInsertPDGFR TMD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A-mCherry (MTK 4a) (pAN3844)
Plasmid#194223PurposeMammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherryDepositorInsertT2A-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)
Plasmid#194229PurposeLentiviral expression vector expressing LCB1 SynNotch and mCherry transduction markerDepositorInsertPGK-LCB1-SynNotch-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTK234_028
Plasmid#123908PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_055
Plasmid#123920PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)
Plasmid#194231PurposeLentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB1-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)
Plasmid#194230PurposeLentiviral expression vector expressing LCB3 SynNotch and mCherry transduction markerDepositorInsertPGK-LCB3-SynNotch-T2A-mCherry
UseLentiviralTagsExpressionMutationE45D mutation in LCB3PromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
Plasmid#194232PurposeLentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB3-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON.BA.5
Plasmid#213071PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intactDepositorInsertpαH-S-RRAR-OMICRON.BA.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685, furin site inta…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK GSC 3'UTR
Plasmid#19859DepositorInsertGSC 3'UTR (GSC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)
Plasmid#194226PurposeLentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction markerDepositorInsertPGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-CBS (WT)
Plasmid#182923PurposeMammalian expression plasmid for human CBSDepositorInsertCystathionine Beta-Synthase (CBS Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-CBS (H65R)
Plasmid#182924PurposeMammalian expression plasmid for human CBS mutant H65RDepositorInsertCystathionine Beta-Synthase (CBS Human)
UseTagseGFPExpressionMammalianMutationchanged histidine 65 to argininePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNANOG-Luc
Plasmid#25900DepositorInsertNANOG promoter (NANOG Human)
UseTagsLuciferaseExpressionMammalianMutationNonePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only