We narrowed to 150,108 results for: ins

1. PB-U6insert-EF1puro

   Plasmid

   #104537

   Purpose

   A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

   Depositor

   George Church

   Article

   Kalhor et al Science. 2018 Aug 9. pii: science.aat980

   Type

   Empty backbone

   Use

   CRISPR, Mouse Targeting, and Synthetic Biology ; …

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-calpainsensor

   Plasmid

   #36182

   Purpose

   detection of activity of ubiquitous calpains

   Depositor

   Isabelle Richard

   Article

   Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

   Insert

   alpha-Fodrin cleavage site (Sptan1 Mouse)
   

   Tags

   eCFP and eYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTMB352_ZFcharm_Kv1_split-inteins

   Plasmid

   #220848

   Purpose

   Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv2
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_EGFR_p.769_770insASV

   Plasmid

   #82880

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Mutation

   ASV insertion between AA 769 and 770

   Promoter

   None

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ins:MCS2; cryaa:RFP

   Plasmid

   #110286

   Purpose

   A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Type

   Empty backbone

   Use

   Zebrafish

   Promoter

   insulin

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTD-NTwinStrep_Sm

   Plasmid

   #45937

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMC1-TwinStrepII

   Plasmid

   #83267

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TMC1 (Tmc1 Mouse)
   

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTD-CTwinStrep

   Plasmid

   #45939

   Purpose

   use to create C-terminal Twin-Strep-tag fusion proteins

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. RINS1(mut)

   Plasmid

   #107291

   Purpose

   Plasmid for the expression of the inactive mutant of RINS1 sensor

   Depositor

   Dmytro Yushchenko

   Article

   Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

   Insert

   RINS1(mut)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   two Arg/Ser mutations at the beginning and in the…

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Promoter

    TRE3G

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTDpelB-C_sfYFPTwinStrep

    Plasmid

    #45944

    Purpose

    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    

    Tags

    PelB export sequence and Twin-Strep-tag

    Expression

    Bacterial

    Promoter

    lacIq/Ptrc

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTDpelB-NTwinStrep

    Plasmid

    #45940

    Purpose

    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis, PelB, and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. proinsulin-EYFP

    Plasmid

    #218952

    Purpose

    Expresses proinsulin fused to EYFP in bacteria

    Depositor

    Benjamin Glick

    Insert

    proinsulin-EYFP
    

    Tags

    proinsulin

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SUMO_TwinStrep_Cas7-11_crRNA

    Plasmid

    #197612

    Purpose

    For protein purification of Cas7-11

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    SUMO_TwinStrep_Cas7-11 with crRNA
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. DFNB31-TwinStrepII

    Plasmid

    #83196

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    DFNB31 (Whrn Mouse)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PDZD7-TwinStrepII

    Plasmid

    #83198

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    PDZD7 (Pdzd7 Mouse)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. USH1CA1-TwinStrepII

    Plasmid

    #83202

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    USH1C (Ush1c Mouse)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. ins:GCaMP6s; cryaa:RFP

    Plasmid

    #110285

    Purpose

    A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Insert

    GCaMP6s
    

    Use

    Zebrafish

    Promoter

    insulin

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CACNA2D1-TwinStrepII

    Plasmid

    #83192

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    CACNA2D1 (Cacna2d1 Rat)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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