We narrowed to 146,489 results for: ins

1. USH1CA1-TwinStrepII

   Plasmid

   #83202

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   USH1C (Ush1c Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINS-UBE3C

   Plasmid

   #66711

   Purpose

   Expresses UBE3C HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KiAA0010 (UBE3C )
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pTDpelB-NTwinStrep

   Plasmid

   #45940

   Purpose

   use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis, PelB, and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. SUMO_TwinStrep_Cas7-11_crRNA

   Plasmid

   #197612

   Purpose

   For protein purification of Cas7-11

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   SUMO_TwinStrep_Cas7-11 with crRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. kanMX6-ins2

   Plasmid

   #195039

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTMB352_ZFcharm_Kv1_split-inteins

   Plasmid

   #220848

   Purpose

   Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv2
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINS-AREL1

   Plasmid

   #66710

   Purpose

   Expresses AREL1 HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KIAA0317 (AREL1 )
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. CACNA2D1-TwinStrepII

   Plasmid

   #83192

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   CACNA2D1 (Cacna2d1 Rat)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ins:GCaMP6s; cryaa:RFP

   Plasmid

   #110285

   Purpose

   A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Insert

   GCaMP6s
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   insulin

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pINS-Blast

    Plasmid

    #31388

    Depositor

    Yegor Vassetzky

    Article

    Dmitriev et al BMC Res Notes. 2008 . 1():135.

    Insert

    bsd
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. InsRA-TagBFP

    Plasmid

    #79791

    Purpose

    Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Use

    Tags

    TagBFP extracellular

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. SLC9A3R2-TwinStrepII

    Plasmid

    #83200

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    SLC9A3R2 (Slc9a3r2 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTD-C_eYFPTwinStrep

    Plasmid

    #45942

    Purpose

    expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic eYFP (codon usage adapted to E.coli K12)
    

    Use

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq/Ptrc

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTD-C_sfYFPTwinStrep

    Plasmid

    #45943

    Purpose

    expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    

    Use

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq/Ptrc

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_EGFR_p.769_770insASV

    Plasmid

    #82880

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    ASV insertion between AA 769 and 770

    Promoter

    None

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTcon2-5INS_16

    Plasmid

    #102292

    Purpose

    Yeast Surface Display of 5Ins_16 scFV

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. natMX6-ins5

    Plasmid

    #195042

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. InsRA-EBFP2

    Plasmid

    #79797

    Purpose

    Insulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Use

    Tags

    EBFP2 extracellular

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLEX_307-SOD1E133insTT

    Plasmid

    #83446

    Purpose

    Mammalian expression of SOD1E133insTT.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133insTT (after GA)

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAPZA1-TwinStrepII

    Plasmid

    #83193

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    CAPZA1 (Capza1 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge