We narrowed to 16,542 results for: mel

1. EGFP-Rab8a-QL

   Plasmid

   #86076

   Purpose

   A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   CMV

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EGFP-Rab8a-TN

   Plasmid

   #86077

   Purpose

   A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed threonine 22 to aspargine

   Promoter

   CMV

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLL3.7m-psMEK1

   Plasmid

   #89361

   Purpose

   A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2017 Feb 24;355(6327):836-842.

   Insert

   psMEK1 (MAP2K1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Human influenza hemagglutinin (HA) tag and pdDron…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MTNR1B-Tango

   Plasmid

   #66444

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   MTNR1B (MTNR1B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

   Plasmid

   #40807

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(44-51), S218E, S222D

   Promoter

   CMV

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

6. L1E-1

   Plasmid

   #21193

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek1 (Esr1, MAP2K1 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Mek1 R4F fused to ER*, constitutive active

   Promoter

   Available since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

7. CC2

   Plasmid

   #21207

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek1 (Esr1, MAP2K1 Mouse, Human)
   

   Use

   Subclone vector

   Tags

   Expression

   Mutation

   Mek1 R4F fused to ER*, constitutive active

   Promoter

   Available since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

8. DN9

   Plasmid

   #21209

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek1 (Esr1, MAP2K1 Mouse, Human)
   

   Use

   Subclone vector

   Tags

   Expression

   Mutation

   Mek1 K97M fused to ER*, catalytically inactive

   Promoter

   Available since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

9. GST-Rab8a-QL

   Plasmid

   #86079

   Purpose

   A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET14b His Rab8a Q67L

    Plasmid

    #186014

    Purpose

    His Rab8a Q67L

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    His Rab8a Q67L (RAB8A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

12. LDN

    Plasmid

    #21195

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (Esr1, MAP2K1 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Mek1 K97M fused to ER*, catalytically inactive

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

13. GST-Rab8a-TN

    Plasmid

    #86080

    Purpose

    A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTNR1B-DuET

    Plasmid

    #213349

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1B (MTNR1B Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV1)

    Viral Prep

    #62723-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent expression of ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato (Cre-dependent)

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral Prep

    #62723-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato (Cre-dependent)

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

17. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

    Viral Prep

    #128589-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] (#128589). In addition to the viral particles, you will also receive purified AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. Syn-driven, Flp-dependent ChrimsonR-tdTomato expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-Syn-Chronos-GFP (AAV Retrograde)

    Viral Prep

    #59170-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Chronos-GFP under the control of the Synapsin promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    GFP

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

19. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

    Viral Prep

    #130909-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    CAG

    Tags

    tdTomato (Flp-dependent)

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pMCL-HA-MAPKK1-8E (K97M)

    Plasmid

    #40811

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K97M

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only