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pCFJ1001

Plasmid

#44476

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, [4-3] Gateway

Use

Gateway Cloning

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ496

Plasmid

#44488

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

500bp Mos1, unc-119, fosmid cassette

Use

Fosmid recombineering cassette

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1002

Plasmid

#44477

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, [4-3] Gateway

Use

Gateway Cloning

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ907

Plasmid

#44473

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, [4-3] Gateway

Use

Gateway Cloning

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ66

Plasmid

#24981

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119 Punc-122::GFP

Use

Gateway Cloning

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ1273

Plasmid

#44487

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ908

Plasmid

#44474

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, [4-3] Gateway

Use

Gateway Cloning

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Expression

Bacterial

Promoter

LacUV5

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1259

Plasmid

#44479

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, [4-3] Gateway

Use

Gateway Cloning

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1200

Plasmid

#44485

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ1000

Plasmid

#44475

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, [4-3] Gateway

Use

Gateway Cloning

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-3p-mCherry-P2A-flag-UltraID

Plasmid

#227174

Purpose

For organ-specific expression of separated mCherry and UltraID in the body-wall muscle and neighboring vulval muscle cells of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

myo-3

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-3p-gfp-halo

Plasmid

#227171

Purpose

For organ-specific expression of GFP-Halo in the body-wall muscle and neighboring vulval muscle cells of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

GFP

Tags

Halotag

Expression

Worm

Promoter

myo-3

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-2p-mCherry-P2A-flag-UltraID

Plasmid

#227172

Purpose

For organ-specific expression of separated mCherry and UltraID in the pharyngeal muscle of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

myo-2

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-ges-1p-mCherry-P2A-flag-UltraID

Plasmid

#227173

Purpose

For organ-specific expression of separated mCherry and UltraID in the intestine of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

ges-1

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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