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Plasmid#200997PurposepLv2-pUC18R6KT-miniTn7-Tc, TcR, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only
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pQF-mNGsacB
Plasmid#203673PurposepQF with mNeonGreen and sacB under control of PQ5DepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQ5Available sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZH520
Plasmid#102673PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH521
Plasmid#102674PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOGG216
Plasmid#114002PurposeLevel 2 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pZH517
Plasmid#102670PurposeTetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah10
Plasmid#122577PurposeMAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)DepositorInserttetQ/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
q4wt
Plasmid#24502DepositorInsertGq alpha
UseTagsHA (see comments)ExpressionMammalianMutationPromoterAvailable sinceMay 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQFT-lacZ
Plasmid#208896PurposepQFT with lacZ under control of PQJDepositorInsertlacZ
UseTagsExpressionBacterialMutationContains two silent mutations and a missense muta…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGC480
Plasmid#42600DepositorInsertlet-363 cDNA
UseRNAiTagsExpressionMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
MXS_rtTA3
Plasmid#62428PurposeMXS_chaining vector with rtTA3DepositorInsertReverse tetracycline transactivator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGL2_137
Plasmid#199084PurposeEven ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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