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pCMV 3xFLAG-LC3A G120A

Plasmid

#123090

Purpose

Expresses 3xFLAG-LC3A G120A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.0

Plasmid

#149429

Purpose

miniTdg gene

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

SNPs of the Tdg gene from the OLA/129 strain

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pVenus-CyclinB1(R42A) (1317)

Plasmid

#39873

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

CyclinB1(R42A) (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

R42A = destruction box mutations = non-destructib…

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
TFORF2600

Plasmid

#144213

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

POU6F2 (POU6F2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-TRC8 513-664

Plasmid

#185352

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

TRC8 (RNF139 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 513-664 only

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-Q927P4

Plasmid

#103131

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS Jaws mVenus_tr

Plasmid

#111553

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-GPR150

Plasmid

#106050

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR150 (GPR150 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-PTPRT (R632X)

Plasmid

#16629

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT R632X (PTPRT Human)

Expression

Mammalian

Mutation

truncated R632X mutant

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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13XLexAop2 IVS Jaws mCherry_tr

Plasmid

#111551

Purpose

Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pME-foxc1a

Plasmid

#132971

Purpose

full-length of foxc1a without stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

foxc1a (foxc1a Zebrafish)

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo-mApg5(K130R)

Plasmid

#22957

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
p6589 MSCV-IP N-HAonly CHEK2

Plasmid

#34903

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHEK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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gh80

Plasmid

#106751

Purpose

expression of gRNA targeting MGAT4B

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT4B (MGAT4B Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-SARA-V5-His6

Plasmid

#67431

Purpose

Expression of hSara with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-LC3A G120

Plasmid

#123091

Purpose

Expresses 3xFLAG-LC3A G120 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 121. Stop codon after G120.

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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MAP1LC3A-V1

Plasmid

#98569

Purpose

Expresses MAP1LC3A-V1

Depositor

Shahenda El-Naggar

Article

Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

Insert

MAP1LC3A (MAP1LC3A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3b(1454/3172)P2P-wt

Plasmid

#21404

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only