We narrowed to 5,308 results for: pcDNA3.1+

1. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169591

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin-2

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. b2-TS3

   Plasmid

   #103877

   Purpose

   full-length human b2 with tension sensor at b2_733

   Depositor

   Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
   

   Use

   Tags

   tension sensor module

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-NES-ZapCY2

   Plasmid

   #59016

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

   Insert

   NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. aL-mEos3.2

   Plasmid

   #113154

   Purpose

   constrained LFA-1-mEos3.2 fusion

   Depositor

   Timothy Springer

   Article

   Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

   Insert

   full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
   

   Use

   Tags

   mEOS3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mcherry Beta 4

   Plasmid

   #36114

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein beta subunit B4 (Gnb4 Mouse)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

6. mcherry Beta 3

   Plasmid

   #36113

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein beta subunit B3 (GNB3 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pPA-RL-FL

   Plasmid

   #78863

   Purpose

   DULIP Tandem Construct used for data normalization

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   Firefly
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-2xFLAG-Atoh1ER-DsRed

   Plasmid

   #101735

   Purpose

   Expresses mouse Atoh1 fused to ER and DsRed

   Depositor

   Albert Edge

   Article

   Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Use

   Tags

   2x FLAG, DsRed, and ER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. deltaPYD-NLRP11.MYC

   Plasmid

   #131197

   Purpose

   mammalian expression

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   deltaPYD-NLRP11 (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   deletion of the pyrin domain (amino acids 1-124 m…

   Promoter

   pCMV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NES-ZapCmR1.1

    Plasmid

    #59014

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pTS1026-Tier1-PhCMV-3xNLS-iRFP670

    Plasmid

    #169532

    Purpose

    Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nucleus-localized far-red fluorescent protein iRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. a5-ecto-pcDNA31hygro

    Plasmid

    #113386

    Purpose

    Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human alpha 5 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFL-V5-GW

    Plasmid

    #78857

    Purpose

    DULIP Prey Destination Vector (Gateway, N-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPA-RL-GW

    Plasmid

    #78855

    Purpose

    DULIP Bait Destination Vector (Gateway, N-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Protein A-Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YFP Gamma 12

    Plasmid

    #36109

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 12 (Gng12 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-BAP-Sox2

    Plasmid

    #133281

    Purpose

    Expression of target proteins BAP-Sox2 in mammalian cells

    Depositor

    Arman Kulyyassov

    Article

    Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Tags

    Biotin Acceptor Peptide contains 7-His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. V5-WT-short-UBASH3A

    Plasmid

    #192088

    Purpose

    Mammalian expression of human wild-type short-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YFP-Gamma 5

    Plasmid

    #36044

    Depositor

    Narasimhan Gautam

    Article

    Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

    Insert

    G protein subunit Gamma 5 (Gng5 Rat)
    

    Use

    Tags

    His and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pActin-miRFP718nano

    Plasmid

    #195745

    Purpose

    Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human beta-actin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGW-FL-V5

    Plasmid

    #78858

    Purpose

    DULIP Prey Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge