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pLenti-VQRRA-P2A-GFP-PGK-Puro

Plasmid

#110864

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZmat3.55.9/Cre

Plasmid

#167853

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80C (1224-1556)

Plasmid

#29445

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1224-1556 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
p-miR-Report + WT miR-181 BS in CDX2 3UTR

Plasmid

#22792

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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p-miR-Report + MT miR-181 BS in CDX2 3UTR

Plasmid

#22793

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Available Since

March 30, 2010

Availability

Academic Institutions and Nonprofits only
pX330_H1.0

Plasmid

#247482

Purpose

Expresses SpCas9 and a sgRNA targeting the human histone H1.0 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Promoter

U6

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pEG-MeCP2_R270X (pc4751)

Plasmid

#248579

Purpose

Mammalian expression plasmid of MeCP2-R294X (aa1- aa293)

Depositor

Cristina Cardoso

Article

Romero et al Commun Biol. 2025 Dec 8. doi: 10.1038/s4

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa1-aa269

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEG-MeCP2_R294X (pc4753)

Plasmid

#248580

Purpose

Mammalian expression plasmid of MeCP2-R294X (aa1- aa293)

Depositor

Cristina Cardoso

Article

Romero et al Commun Biol. 2025 Dec 8. doi: 10.1038/s4

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa1-aa293

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-MeCP2-R168X (pc4734 )

Plasmid

#249081

Purpose

Bacteria expression plasmid of MeCP2-R168X (aa1-aa167)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Expression

Bacterial

Mutation

MeCP2-R168X (aa1-aa167)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-MeCP2-R255X (pc4735)

Plasmid

#249082

Purpose

Bacteria expression plasmid of MeCP2-R255X (aa1-aa254)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Expression

Bacterial

Mutation

MeCP2-R255X (aa1-aa254)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2-R168X (pc4742 )

Plasmid

#249084

Purpose

Bacteria expression plasmid of GFP tagged MeCP2-R168X (aa1-aa167)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Mutation

MeCP2-R168X (aa1-aa167)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2-R255X (pc4743 )

Plasmid

#249085

Purpose

Bacteria expression plasmid of GFP tagged MeCP2-R255X (aa1-aa254)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Mutation

MeCP2-R255X (aa1-aa254)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgPTPN11-1

Plasmid

#253090

Purpose

CRISPRi single-guide against PTPN11

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PTPN11 (PTPN11 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-dTomato-WPRE-bGHpA

Plasmid

#231019

Purpose

For strong lentivirus-mediated expression of dTomato

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

dTomato

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-shMfn1-mCherry-CAAX

Plasmid

#228740

Purpose

Membrane-tethered mCherry shRNA targeting Mfn1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Mfn1 (Mfn1, Mfn1 Rat, Mouse)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstar2

Plasmid

#241272

Purpose

Expresses NADPH/NADP+ biosensor NAPstar2 in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar2

Expression

Plant

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstar7

Plasmid

#241224

Purpose

Expresses NADPH/NADP+ biosensor NAPstar7 in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar7

Expression

Plant

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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