We narrowed to 10,667 results for: plasmids 101

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ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Expression

Bacterial

Promoter

lac promoter

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1wt-IRES-GFP

Plasmid

#33333

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1ER-IRES-GFP

Plasmid

#33335

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

ER

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pGL2-basic mt E2F-luciferase

Plasmid

#133708

Purpose

Expression of E2F1-mt-luc.

Depositor

James A. DeCaprio

Article

Zalvide et al Mol Cell Biol 1998 Mar;18(3):1408-15.

Insert

E2F1 (E2F1 Human)

Use

Trxn factor bind site

Expression

Mammalian

Mutation

the three binding site copies are mutated form

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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KCNE1_pCSdest

Plasmid

#53871

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

KCNE1 (KCNE1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-Syn-FLEX-rc [ChromeT-GFP]

Plasmid

#153539

Purpose

AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBG1

Plasmid

#111696

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBG1 (TUBG1 Human)

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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DSCR6_pCSdest

Plasmid

#53815

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RIPPLY3 (DSCR6 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pPB_TRE3G_scFV-sfGFP-G9aCD

Plasmid

#235580

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

G9a (Ehmt2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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TurboID-CRaf

Plasmid

#226168

Purpose

Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-CRaf (RAF1 Human)

Use

Lentiviral

Tags

EGFP-IRES-BlastR and Myc-TurboID

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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STR-KDEL_PGRN-SBP-mCherry

Plasmid

#180439

Purpose

Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

Depositor

Shawn Ferguson

Article

Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

Insert

Progranulin (GRN Human)

Tags

SBP-mCherry

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Tags

HIS-HA-CMYC

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_K700E_hSF3B1

Plasmid

#176591

Purpose

Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-DNMT3a-DNMT3l

Plasmid

#154140

Purpose

Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP

Tags

HA and sfGFP

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2

Plasmid

#123618

Purpose

Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR_RAF1_94

Plasmid

#111826

Purpose

Knockout Raf1

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting RAF1 94-114 (RAF1 Human)

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChromeQ-GFP]

Plasmid

#153540

Purpose

AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_FLAG_hSF3B1

Plasmid

#176590

Purpose

Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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