We narrowed to 16,618 results for: puromycin

1. pLentiGuide_GFP_NT135

   Plasmid

   #183118

   Purpose

   pLentiGuide modified to express GFP and a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. V51 pIRESpuro-GLUE (pGLUE)

   Plasmid

   #15100

   Purpose

   tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Type

   Empty backbone

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. p6872 pHAGE-N-V5-MAML1-FL

   Plasmid

   #37048

   Depositor

   Peter Howley

   Article

   Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

   Insert

   pHAGE-N-V5-MAML1-FL (MYLIP Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMVt

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ-EGFP:Ajuba

   Plasmid

   #108229

   Purpose

   Lentiviral vector for Tet-inducible Ajuba expression

   Depositor

   Kenneth Irvine

   Article

   Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

   Insert

   AJUBA (AJUBA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Tet/ON Minimal CMV promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

   Plasmid

   #233445

   Purpose

   PiggyBac donor plasmid to overexpress a full length ADAR2

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2-T2A-PuroR-P2A-BFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1a

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin beta1-tev-BHCys

   Plasmid

   #27314

   Depositor

   Timothy Springer

   Article

   Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

   Insert

   integrin beta1 (ITGB1 Human)
   

   Expression

   Mammalian

   Mutation

   integrin beta1(1-708)+GG+TEV+GGKN+BHCys

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

8. SpdCas9 KRAB

   Plasmid

   #188499

   Purpose

   Expresses FLAG tagged SpdCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MyoD-pCLBabe

   Plasmid

   #20917

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   MyoD (Myod1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    No promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZLCv2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106357

    Purpose

    Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG and HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P shSOD2

    Plasmid

    #102976

    Purpose

    Suppression of SOD2

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD2 (SOD2 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-puro-mTwist

    Plasmid

    #1783

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-SIRT6

    Plasmid

    #102325

    Purpose

    Human SIRT6 for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. P2A-NLS-tdtomato-EPG backbone

    Plasmid

    #215548

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe-Dvl2-GFP

    Plasmid

    #109148

    Purpose

    Stable Dvl2 expression

    Depositor

    Mariann Bienz

    Article

    Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

    Insert

    Dvl2 (DVL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-CAG-Brainbow3.2-PGK-PuroTK

    Plasmid

    #159928

    Purpose

    Brainbow transgenesis

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

    Inserts

    NFPnls
    mOrange2
    EGFP
    mKate2
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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