We narrowed to 10,117 results for: Ada

1. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

   Plasmid

   #159803

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Gal4-PGC1 alpha

   Plasmid

   #8892

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pMito-miRFP718nano

   Plasmid

   #195754

   Purpose

   Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   Human cytochrome C oxidase subunit VIII-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiCRISPR v2-sgLKB1 clone3

   Plasmid

   #162127

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-HA-KLF5-his

   Plasmid

   #40904

   Depositor

   Jin-Tang Dong

   Article

   Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

   Insert

   Kruppel-like factor 5 (KLF5 Human)
   

   Use

   Tags

   HA and His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH2B-miRFP718nano

   Plasmid

   #195750

   Purpose

   Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   H2B-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV103

   Plasmid

   #63193

   Purpose

   pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Worm and Yeast

   Mutation

   Promoter

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSBbi_RP_MicroID2-FLAG-NES

   Plasmid

   #188525

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3xFLAG-CMV14-mULK1

    Plasmid

    #24301

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

11. qTAG-AAVS1-Ef1a-Zeo

    Plasmid

    #227268

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAC_N_GCM1

    Plasmid

    #167759

    Purpose

    Expresses GCM1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    GCM1 (GCM1 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. opto-Tiam1

    Plasmid

    #174508

    Purpose

    Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Tiam1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

    Plasmid

    #200401

    Purpose

    Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Open-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-HA C/EBP gamma

    Plasmid

    #15715

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    C/EBP gamma (CEBPG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pHAGE-FKBP-GFP-SINTBAD

    Plasmid

    #216840

    Purpose

    Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only