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pTRIPZ-DoxOn-shCUX1-5150

Plasmid

#90469

Purpose

Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-rev-g2-2A-Venus

Plasmid

#71410

Purpose

This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

Depositor

William Wisden

Article

Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

Inserts

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

mouse hdc gene promoter, works as a pan neuronal …

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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GAL FLAG46QDProCFPp303

Plasmid

#15585

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
BirA_BVES untagged

Plasmid

#196581

Purpose

expresses BirA

Depositor

Cheryl Arrowsmith

Article

Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

Insert

BirA_Ecoli

Use

Baculovirus expression

Available Since

June 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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FLAG-EAF pBacPAK8

Plasmid

#15323

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

6x His and FLAG

Promoter

polyhedrin

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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GPD 25QDProGFP p416

Plasmid

#15568

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pTRIPZ shCUX1-5328

Plasmid

#100815

Purpose

Lentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA

Use

Lentiviral

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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attB-P7-attP-YFP

Plasmid

#195571

Purpose

A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

Depositor

Richard Murray

Article

Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

Insert

Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream

Use

Synthetic Biology

Promoter

P7

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GAL FLAG72QDProCFPp303

Plasmid

#15586

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGEM-myr-eGFP-SV40pA-FKF

Plasmid

#119866

Purpose

Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Tags

2x myristoylation

Expression

Bacterial

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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GPD 104QDProGFP p416

Plasmid

#15569

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 104Q Delta Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL2 pBacPAK8

Plasmid

#15327

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

Eleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
MTK3_001

Plasmid

#123952

Purpose

Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3XNLS::CRY2PHR::p65

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-EAF2 pBacPAK8

Plasmid

#15326

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor 2 (EAF2 Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
MTK0_012

Plasmid

#123967

Purpose

Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hRosa26-BxBI landing pad

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MET FLAG103QDProCFPp303

Plasmid

#15591

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mm

Plasmid

#51059

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMHBAD14

Plasmid

#42304

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaE C-intein

Tags

GB1-H6

Expression

Bacterial

Promoter

araBAD

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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