We narrowed to 14,906 results for: Tet-on

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

Plasmid

#184395

Purpose

Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

Plasmid

#184395

Purpose

Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

Plasmid

#78099

Purpose

Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

Histone H2B - Citrine (H2BC21 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

5xTetO pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

Plasmid

#78099

Purpose

Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

Histone H2B - Citrine (H2BC21 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

5xTetO pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

Plasmid

#184396

Purpose

Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

Plasmid

#184396

Purpose

Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

Plasmid

#68347

Purpose

AAV plasmid expressing the FlpO recombinase. with empty gRNA

Depositor

Jannik Elverløv-Jakobsen

Insert

FlPO

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

Plasmid

#68347

Purpose

AAV plasmid expressing the FlpO recombinase. with empty gRNA

Depositor

Jannik Elverløv-Jakobsen

Insert

FlPO

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

Plasmid

#184398

Purpose

Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

Plasmid

#184398

Purpose

Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

Plasmid

#78353

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

Plasmid

#78353

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge