We narrowed to 4,482 results for: ARA-2

1. Str-Endo-KKXX_SBP-EGFP-LIMP2

   Plasmid

   #222323

   Purpose

   Synchronize the trafficking of LIMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEN25-Ub Hs

   Plasmid

   #83232

   Purpose

   pHis-par2-Ub Hs

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Ubiqutin (UBC Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-shLuc.mKO2

   Plasmid

   #85224

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-HA-CAD S1900A

   Plasmid

   #46240

   Purpose

   FLAG-HA-CAD with S1900 mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1900 changed to Alanine (S1900A)

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32bplus_trx_dAChE4

   Plasmid

   #83917

   Purpose

   Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli

   Depositor

   Sarel Fleishman

   Article

   Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

   Insert

   Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
   

   Use

   Tags

   His, S-tag, and TRX

   Expression

   Bacterial

   Mutation

   V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…

   Promoter

   T7 promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-hPIKfyve

   Plasmid

   #121148

   Purpose

   Expresses GFP-tagged human PIKfyve in mammalian cells.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

   Insert

   1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEos3.2FPP

   Plasmid

   #203758

   Purpose

   Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   HRas c-term (HRAS Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A

   Plasmid

   #238337

   Purpose

   proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Use

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-H505A-H741A-H975A

   Promoter

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A

    Plasmid

    #238333

    Purpose

    proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-R372A-H373A-R376A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A

    Plasmid

    #238338

    Purpose

    proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-P378V-G500V-G501A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A

    Plasmid

    #238339

    Purpose

    proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-R722S-D724A-T726A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A

    Plasmid

    #238340

    Purpose

    proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-K383A-K447A-K598A-R722A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A

    Plasmid

    #238334

    Purpose

    proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-R372A-H373A-R376A-E882A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A

    Plasmid

    #238341

    Purpose

    proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-K379A-K381A-K498A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T1_GST-GBRP

    Plasmid

    #223728

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-116 only, with Glycine exposed

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCOG40-HRS (257-276) Hs

    Plasmid

    #83236

    Purpose

    pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    HRS (257-276) Hs (HGS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    258-277

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid

    #49060

    Purpose

    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP (chloride-sensitive)

    Expression

    Mammalian

    Mutation

    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-EVA1ext-hFcIgG3-IRES-YFP

    Plasmid

    #64926

    Purpose

    To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

    Depositor

    Pau Serra

    Article

    Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

    Insert

    mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Human, Mouse)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid

    #49061

    Purpose

    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …

    Promoter

    CMV

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge