We narrowed to 4,101 results for: GAST
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Plasmid#113009PurposeFor bacterial expression of MBP fusion of N terminal region of Drosophila TralDepositorAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only
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pMAL c2x Tral FL-15A
Plasmid#113011PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)DepositorInsertfull length tral (tral Fly)
ExpressionBacterialMutation15 PNG phosphorylation sites mutated to AAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C
Plasmid#113008PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila TralDepositorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C 15A
Plasmid#113007PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)DepositorInsertC terminus of tral (tral Fly)
ExpressionBacterialMutationPNG phosphorylation sites mutated to AAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4D03
Plasmid#70969PurposeGateway entry cloneDepositorInsertTaf12 (Taf12 Fly)
UseGateway entry vectorAvailable SinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1F03
Plasmid#71071PurposeGateway entry cloneDepositorInsertSu(var)205 (Su(var)205 Fly)
UseGateway entry vectorAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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BII-CMV-AfmW3A
Plasmid#133402PurposePiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cellsDepositorAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFOSGE_COF4C03
Plasmid#70961PurposeGateway entry cloneDepositorInsertHis2Av (His2Av Fly)
UseGateway entry vectorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-g-osk-TRICK
Plasmid#71244Purposeoskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.DepositorInsertoskar (osk Fly)
UseP element-mediated germline transformationTags12xPP7 and 6xMS2ExpressionInsectPromoteroskAvailable SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-EGFP-Actin5c
Plasmid#15312DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA
Plasmid#211818PurposeAAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNADepositorInsertC-terminus of SpCas9-ABE8e and tracrRNA
UseAAVExpressionMammalianPromoterchicken β-actin promoter and U6 promoterAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only