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pMXs-GATA2

Plasmid

#131602

Purpose

expression of human GATA2 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

GATA2 (GATA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Y1175F-VEGFR2-mCherry

Plasmid

#139608

Purpose

Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CHKA

Plasmid

#218540

Purpose

human CHKA cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-p53 2KR

Plasmid

#78799

Purpose

To overexpress p53 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

p53 (TP53 Human)

Tags

HA

Expression

Mammalian

Mutation

K291R, K292R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pCDH-CMV-HA-CFP-SLMAP (isoform r)

Plasmid

#235684

Purpose

Express CFP tagged SLMAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SLMAP (SLMAP Human)

Use

Lentiviral

Tags

HA-CFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pcDNA3

Plasmid

#206072

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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LUC05 (L/Cys/P)

Plasmid

#112146

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)

Expression

Mammalian

Mutation

none

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3 R186S

Plasmid

#175779

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Mutation

R186S

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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ACTN3-pEGFP

Plasmid

#52670

Purpose

Overexpression of GFP-tagged α-actinin-3

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

ACTN3 (ACTN3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA -CLCN3-3xFlag

Plasmid

#225506

Purpose

CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmReNL

Plasmid

#182970

Purpose

Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmReNL

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAX APPs-695-alpha

Plasmid

#30147

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Expression

Mammalian

Mutation

amino acids 1–612

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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p51.SRE.mCMV.CD63-GFP.wpre

Plasmid

#226773

Purpose

CD63-GFP in p52 (SRE backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

CD63 (CD63 )

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L861Q)

Plasmid

#32068

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L861Q

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only