We narrowed to 14,779 results for: sta

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MSCV Puro-UBC:GFP

Plasmid

#68482

Purpose

Retrovirus expressing GFP driven by UBC promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and UBC

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Publication

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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T7 promoter + filler DNA reporter plasmid

Plasmid

#156454

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Publication

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM165/166-48nt

Plasmid

#84091

Purpose

target miRNA165/166 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Publication

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM165/166

Expression

Plant

Promoter

2x35S

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Publication

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Expression

Bacterial

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLG021 (AVAST type 5)

Plasmid

#157899

Purpose

Expresses the AVAST type 5 defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 5 (STAND ATPase with N-terminal SIR2)

Expression

Bacterial

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG NUFIP1

Plasmid

#110952

Purpose

Transient expression of FLAG NUFIP1

Depositor

David Sabatini

Publication

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 (NUFIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Publication

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAK beta

Plasmid

#141195

Purpose

Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Publication

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 isoform 2 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSEMS-SDHB-Halo7Tag

Plasmid

#108973

Purpose

Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

Depositor

Karin Busch

Publication

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)

Tags

Halo7-tag

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKan-PRFA1-9myc-AID*(N)

Plasmid

#99528

Purpose

N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

RFA1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*

Plasmid

#99515

Purpose

C-terminal AID* degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Mutation

internal inversion of nucleotides 265-312 of the …

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP-GFP-shch-TOG

Plasmid

#69113

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Publication

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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yWL268 MCP-GFP

Plasmid

#197052

Purpose

pUBC-NLS-HA-2xMCP-2xGFP

Depositor

Robert Singer

Publication

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-2xGFP

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.A184V

Plasmid

#106202

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Publication

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-TIE1

Plasmid

#23946

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TIE1 (TIE1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
M13-kmR

Plasmid

#206861

Purpose

M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

Depositor

Irene Chen

Publication

Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

Insert

Kanamycin resistance gene

Expression

Bacterial

Promoter

lac

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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