We narrowed to 6,725 results for: SIM;

1. pAT9784-xABE

   Plasmid

   #163005

   Purpose

   plasmid expressing xABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   xABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO chicken-cPLA2-C2-domain

   Plasmid

   #170753

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

   Insert

   PLA2G4A-C2 domain (PLA2G4A Chicken)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-rsAKARev(T/A)

   Plasmid

   #166232

   Purpose

   Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

   Depositor

   Peter Dedecker

   Article

   Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

   Insert

   Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 506 in rsAKARev to Alanine

   Promoter

   CMV

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. OfCasp3b

   Plasmid

   #166054

   Purpose

   a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

   Depositor

   Clay Clark

   Article

   Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

   Insert

   Orbicella faveolata caspase-3b
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PaCasp3

   Plasmid

   #166052

   Purpose

   a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

   Depositor

   Clay Clark

   Article

   Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

   Insert

   Porites astreiodes caspase-3
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK234_058

   Plasmid

   #123984

   Purpose

   Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sg Safetargeting Chr11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK234_038

   Plasmid

   #123917

   Purpose

   Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA-pSV40
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK234_043

   Plasmid

   #123913

   Purpose

   Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA-pSV40
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ2365 - Intron GG1 - 250bp no PATC

   Plasmid

   #159883

   Purpose

   Golden Gate compatible intron with no PATCs for negative controls

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 250bp no PATC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ2345 - Intron GG2 - 250bp no PATC

    Plasmid

    #159884

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ2366 - intron GG3 - 250bp no PATC

    Plasmid

    #159885

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG3 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EcNusGKOW

    Plasmid

    #113124

    Purpose

    Expresses C-terminus of E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-124

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EcRfaHKOW

    Plasmid

    #113125

    Purpose

    Expresses C-terminus of E. coli RfaH

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQP-2376

    Plasmid

    #138974

    Purpose

    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCk4

    Plasmid

    #136698

    Purpose

    Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MTK234_015

    Plasmid

    #123991

    Purpose

    Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hROSA26 sg4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK8_005

    Plasmid

    #123885

    Purpose

    Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    F1-KanR-ColE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK0_021

    Plasmid

    #123936

    Purpose

    Encodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TUL3R4 spacer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK234_047

    Plasmid

    #124055

    Purpose

    Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK234_048

    Plasmid

    #124056

    Purpose

    Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge