We narrowed to 166,040 results for: addgene

1. eSpCas9(1.1)

   Plasmid

   #71814

   Purpose

   Expresses high specificity SpCas9. Px330-like plasmid.

   Depositor

   Feng Zhang

   Article

   Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

   Has Service

   Cloning Grade DNA

   Insert

   enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRG (Lenti_sgRNA_EFS_GFP)

   Plasmid

   #65656

   Purpose

   Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter-driven sgRNA expression and EFS promo…

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ColorfulCell

   Plasmid

   #62449

   Purpose

   MXS_chaining vector with 6 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::GTS-Citrine-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::NLS-TagBFPx3-bGHpA
   CMV::MTS-AzamiGreen-bGHpA
   CMV::iRFP670-SKL-bGHpA
   CMV::ETS-mCherry-KDEL-bGHpA
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCW-Flag-CtIP

   Plasmid

   #71108

   Purpose

   mammalian expession of Flag-CtlP

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pIVT-Cas9-TMV

   Plasmid

   #238016

   Purpose

   for DNA-free gene knockout editing in rice and wheat or other plants

   Depositor

   Caixia Gao

   Article

   Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

   Insert

   SpCas9
   

   Expression

   Plant

   Promoter

   T7

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW-Flag-CtIP-T847E

   Plasmid

   #71110

   Purpose

   mammalian expession of Flag-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV-G12-CheRiff-T2A-mCherry

    Plasmid

    #170275

    Purpose

    CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    CheRiff-T2A-mCherry
    

    Use

    AAV

    Promoter

    11xUAS with TATA-box minimal promoter

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGH020_sgRNA_G418-GFP

    Plasmid

    #85405

    Purpose

    hu6 driven sgRNA vector with G418 and GFP selectable markers

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    G418 resistance and GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-EGFP-NLS

    Plasmid

    #165441

    Purpose

    Expresses nuclear EGFP from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    EGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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